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棉花黄萎病菌培养滤液对棉幼苗、种子发芽及离体培养物的毒害作用
引用本文:姚明镜,张献龙,刘金兰,孙济中. 棉花黄萎病菌培养滤液对棉幼苗、种子发芽及离体培养物的毒害作用[J]. 棉花学报, 1994, 0(3)
作者姓名:姚明镜  张献龙  刘金兰  孙济中
作者单位:华中农业大学
基金项目:国家自然科学基金,国际科学基金
摘    要:用试验证实了Czapek's液体培养基培养棉花黄萎病菌所得病菌培养滤液,能使棉苗发病萎蔫甚至干枯死亡;测试了病菌培养滤液对种子发芽、根的生长、愈伤组织诱导、愈伤组织生长及原生质体活力的影响,抗感不同品种表现出较明显的差异。为病菌培养滤液应用于抗病资源的快速筛选与鉴定、体细胞抗性突变体的筛选等研究提供了可靠的参考资料。

关 键 词:棉花黄萎病,病菌培养滤液,离休培养物

Effect of Verticillium dahliae Culture Filtrate on Seedling,Seed Ger-mination and Culture of Cotton in Vitro
Yao Mingjing ,Zhang Xianlong, Liu Jinlan ,Sun Jizhong. Effect of Verticillium dahliae Culture Filtrate on Seedling,Seed Ger-mination and Culture of Cotton in Vitro[J]. Cotton Science, 1994, 0(3)
Authors:Yao Mingjing   Zhang Xianlong   Liu Jinlan   Sun Jizhong
Abstract:The pathomycete of cotton Vedicillium wilt was inoculated and cultured in Czapek's liquid medium,and the culture filtrate were confirmed to cause seedling wilt and dead.Effectof the filtrate on seed germination,callus induction and growth,viability of protoplast weretested.The results showed that the degree of damage differed between resistant and sensitivecultivars. The possible application of pathomycete culture filtrates on the selection of resistantresources and reststant mutants in vitro was disccused.
Keywords:Verticillium dahliae  culture filtrate  cotton culture in vitro
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