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LaCl3和CPZ对磷饥饿番茄液泡膜H+-ATPase和H+-PPase活性的影响
引用本文:徐函兵,曾镭,单树花,刘晶茹,宋克敏.LaCl3和CPZ对磷饥饿番茄液泡膜H+-ATPase和H+-PPase活性的影响[J].北方园艺,2011(7):1-4.
作者姓名:徐函兵  曾镭  单树花  刘晶茹  宋克敏
作者单位:信阳农业高等专科学校,安徽,信阳,464000;华中农业大学,植物科技学院,湖北,武汉,430070;信阳农业高等专科学校,安徽,信阳,464000;华中农业大学,植物科技学院,湖北,武汉,430070;太原师范学院,山西,太原,030031;华中农业大学,植物科技学院,湖北,武汉,430070
基金项目:国家自然科学基金资助项目
摘    要:采用水培法研究了LaCl3和氯丙嗪(CPZ)对磷饥饿番茄幼苗液泡膜H+-ATPase和H+-PPase活性的影响。结果表明:LaCl3处理抑制了液泡膜H+-ATPase活性,但提高了H+-PPase活性;而CPZ处理既抑制了液泡膜H+-ATPase活性,又抑制了液泡膜H+-PPase活性。同时,CPZ处理引起磷饥饿番茄幼苗可溶性蛋白质含量下降,LaCl3处理引起可溶性蛋白质含量增加。

关 键 词:液泡膜  H+-ATPase  H+-PPase  磷饥饿  番茄

Effects of LaCl3 and CPZ on Tonoplast H+-ATPase and H+-PPase Activities in Tomato Seedlings During Phosphate Ptarvation
XU Han-bing,ZENG Lei,SHAN Shu-hua,LIU Jing-ru,SONG Ke-min.Effects of LaCl3 and CPZ on Tonoplast H+-ATPase and H+-PPase Activities in Tomato Seedlings During Phosphate Ptarvation[J].Northern Horticulture,2011(7):1-4.
Authors:XU Han-bing  ZENG Lei  SHAN Shu-hua  LIU Jing-ru  SONG Ke-min
Institution:1.Xinyang Agricultural College,Xinyang,Anhui 464000;2.College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070;3.Taiyuan Normal University,Taiyuan,Shanxi 030031)
Abstract:The effect of calmodulin antagonist chlorpromazine(CPZ) and an inhibitor of Ca2+ channel in plasma membrane La3+ on the activities of tonoplast H+-ATPase and H+-PPase of tomato seedlings under phosphate starvation were studied.The results showed that under phosphate starvation,the activities of tonoplast H+-ATPase and H+-PPase were increased in the roots of tomato seedlings.But this enhancement was abolished by CPZ.Compared with CPZ,treatment with La3+ decreased the activity of H+-ATPase and enhanced H+-PPase activity.Furthermore,CPZ caused the content of soluble proteins decreased but LaCl3 caused soluble increased in the seedlings during phosphate starvation.
Keywords:H+-ATPase  H+-PPase
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