猪PLSCR4基因外显子区PCR-RFLP遗传多态性分析

本研究利用PCR-RFLP技术对我国6个地方猪种、中国野猪以及杜洛克、约克夏和长白3个国外猪品种,共307头猪的磷脂爬行酶基因(PLSCRs)外显子区的遗传变异进行了研究。结果表明:PLSCR4第7外显子处存在T68C的同义突变,第8外显子处存在G4A的错义突变;在T68C突变位点处,杜洛克、约克夏、长白猪、二花脸、五指山和民猪6个猪群优势等位基因为T,荣昌和野猪群体中优势等位基因为C;在G4A突变位点处,杜洛克、约克夏、长白猪、二花脸、五指山和民猪群体中优势等位基因为G,藏猪、荣昌、金华及野猪群体中优势等位基因A。

Genetic Diversity of Exon Regions of Phospholipid Scramblase Gene among Different Pig Breeds

The exons of PLSCR4 gene were analyzed in 307 pigs including six Chinese native pig breeds,Chinese wild boar and three exotic pig breeds(Duroc,Yorkshire and Landrace) by means of PCR-RFLP technology.The results showed that the T68C transition mutation within exon 7 of PLSCR4 was a synonymous mutation and the G4A transition mutation within exon 8 was a missense mutation;The genetic variations were found among all the pig samples at the T68C site of the exon 7,T was the dominant allele in Duroc,Yorkshire,Landrace,Erhualian,Wuzhishan and Min and C was the dominant allele in Rongchang and Chinese wild boar.At the G4A site of exon 8,G was dominant allele in Duroc,Yorkshire,Landrace,Erhualian,Wuzhishanw and Min and A was dominant allele in tibet pig,Rongchang,Jinhua and Chinese wild boar.