| 细胞外基质蛋白mindin的原核表达及对日本血吸虫Sjc23抗原的免疫增强作用研究 |
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| 引用本文： | 孙晓敬,张旭,彭帅,姜宁,陈启军. 细胞外基质蛋白mindin的原核表达及对日本血吸虫Sjc23抗原的免疫增强作用研究[J]. 动物医学进展, 2011, 0(12): 75-79 |
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| 作者姓名： | 孙晓敬 张旭 彭帅 姜宁 陈启军 |
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| 作者单位： | 吉林大学人兽共患病研究所人兽共患病研究教育部重点实验室 |
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| 摘 要： | 根据GenBank中公布的鼠源spon2基因序列,合成全长基因片段,用BamH Ⅰ和Hin dⅢ双酶切后将目的片段插入表达载体pET-22b(+)中,构建pET-22b-mindin重组质粒,并转入大肠埃希菌BL21(DE3)后用IPTG进行诱导表达,产物经SDS-PAGE、Western blot进行检测.将表达蛋白...
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| 关 键 词： | 细胞外基质 mindin Sjc23 原核表达 酶联免疫吸附试验 |
Prokaryotic Expression of the Extracellular Matrix Protein Mindin and Its Immune-enhancing Effect on Schistosoma japonicum |
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| SUN Xiao-jing,ZHANG Xu,PENG Shuai,JIANG Ning,CHEN Qi-jun. Prokaryotic Expression of the Extracellular Matrix Protein Mindin and Its Immune-enhancing Effect on Schistosoma japonicum[J]. Progress In Veterinary Medicine, 2011, 0(12): 75-79 |
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| Authors: | SUN Xiao-jing ZHANG Xu PENG Shuai JIANG Ning CHEN Qi-jun |
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| Affiliation: | (Key Laboratory of Zoonosis,Institute of Zoonosis,Jilin University,Changchun,Jilin,130062,China) |
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| Abstract: | The immune-enhancing effect of mindin was studied.The Mus musculus spon2 gene encoding mindin was synthesized with restriction sites(BamHI、HindⅢ) at 5′and 3′end respectively.The gene fragment was cloned into the expression vector Pet-22b and the HIS-tagged recombinant protein was generated and confirmed with Western blot.Mice were immunized with Sjc23 of Schistosoma japonicum in-combination with HIS-mindin.Specific to antibody responses to Sjc23 in serum was studied by ELISA.The results showed that recombinant mindin can be generated with E.coli BL21(DE3) cell lines.mindin had similar adjuvancity as the classical Freund adjuvant with respect to antibody responses to the Sjc23 antigen of S.japonicum.Thus the study provided the basis for further investigation on the application of mindin as an immune-enhancing agent in vaccine development. |
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| Keywords: | extracellular matrix mindin Sjc23 prokaryotic expression ELISA |
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