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| 北沙参SRAP分子标记体系的建立与优化 | |
| 引用本文： | 齐树杰,沈 镝,李 颖,张钦德,李庆典.北沙参SRAP分子标记体系的建立与优化[J].中国农学通报,2009,25(24):73-77. |
| 作者姓名： | 齐树杰 沈 镝 李 颖 张钦德 李庆典 |
| 作者单位： | 1. 青岛农业大学,山东,青岛,266109 2. 中国农业科学院,北京,100850 3. 山东中医药高等专科学校,山东,烟台,264000 |
| 基金项目： | 国家中医药局课题"北沙参道地药材质量标准规范化研究" |
| 摘 要： | 为北沙参的遗传多样性分析提供一种科学的途径。采用SRAP标记技术，以北沙参基因组DNA为模板，优化了SRAP反应体系的各主要参数。建立了稳定可靠的SRAP-PCR反应体系；20μL反应体系中，DNA的量为80ng、Mg2+ 2.5mmol/L、dNTPs 0.25mmol/L、TaqDNA聚合酶为1U、正反向Primer浓度均为0.1μmol/L。该体系适合北沙参遗传多样性分析、遗传图谱构建等研究。 |
| 关 键 词： | 粮草间作 粮草间作 坡耕地 培肥保土 效益 |
| 收稿时间： | 2009-08-13 |
| 修稿时间： | 2009-09-03 |
Establishment and Optimization of SRAP Reaction System in Glehnia littoralis |
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| Qi Shujie,Shen Di,Li Ying,Zhang Qinde,Li Qingdian.Establishment and Optimization of SRAP Reaction System in Glehnia littoralis[J].Chinese Agricultural Science Bulletin,2009,25(24):73-77. | |
| Authors: | Qi Shujie Shen Di Li Ying Zhang Qinde Li Qingdian |
| Institution: | Qi Shujie, Shen Di, Li Ying, Zhang Qinde, Li Qingdian (1Qingdao Agricultural University, Qingdao Shandong 266109; 2Chinese Academy of Agricultural Sciences, Beijing 100850; 3Shandong College of Traditional Chinese Medicine, Yantai Shandong 264000) |
| Abstract: | To develop a sicentific method for Glehnia litteralis genetic diversity analysis. Glehnia litteralis DNA was used to optimize SRAP system’s main parameters. A stability and reliable SRAP-PCR system was established. In the 20μL system, DNA 80 ng, Mg2+ 2.5 mmol/L, dNTP 0.25mmol/L, Taq 1U, Upstream and Downstream primers both for 0.25μmol/L. The reaction system was suitable to study Glehnia litteralis genetic diversity analysis and gene mapping. |
| Keywords: | Glehnia litteraliszz SRAPzz molecular markerzz system optimizationzz |
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