黑龙江省大豆根腐病致病病原种类分布及抗病种质鉴定

为了明确黑龙江省大豆根腐病菌种类并筛选抗病种质资源，2008年以来，从黑龙江省大豆产区100个地点采集根腐病株，通过采用PDA培养基分离镰刀菌、立枯丝核菌、腐霉菌，选择培养基分离疫霉菌的方法。分离结果表明，各主要致病菌出现频率为：镰刀菌42.7%，腐霉菌25.2%，立枯丝核菌7%，疫霉菌25.2%。对1249份大豆材料在自然发病条件下鉴定的结果表明，病情指数15%以下的材料有471份，占鉴定材料的37.7%；对471份材料人工接种出现频率最高的镰刀菌的结果表明，病情指数15%以下的材料69份，占供试材料的14.6%。通过试验研究明确了大豆根腐病致病病原菌种类；明确了现行大豆品种和资源中有抗根腐病的材料。

Pathogenic Pathogen Categories Distribution and Germplasm Resistance Identification of Soybean Root Rot in Heilongjiang Province

To identify pathogenic pathogen categories of soybean root rot and screen germplasm resistance in Heilongjiang Province, we separated Fusarium oxysporum, Rhizoctonia solani and Pythium debaryanum using PDA culture medium from soybean root rot plants collecting from 100 sites of soybean production areas in Heilongjiang from 2008, and Phytophthora sojae was separated by selective medium. The separation results indicated that, the occurrence frequency of major pathogenic bacterium was, Fusarium oxysporum occupied 42.7% , Pythium debaryanum 25.5% , Rhizoctonia solani 7% and Phytophthora sojae 25.2% . Our results showed that, after identifying 1249 portions of soybean materials infected under natural invasion condition, disease index of 471 portions were under 15%, occupied 37.7% of identified materials. Fusarium oxysporum was more frequent than others in artificial vaccination to 471 portions materials, in which disease index of 69 portions was under 15% and occupied 14.6% of test materials. From our results, we draw the conclusions that, we identified pathogenic pathogen categories of soybean root rot and there were materials resistant to root rot in soybean cultivars and resources.