| 利用基因组发掘技术指导链霉菌SH-62中活性天然产物的分离及鉴定 |
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| 引用本文： | 鲁洲,周俊,何璟.利用基因组发掘技术指导链霉菌SH-62中活性天然产物的分离及鉴定[J].湖北农业科学,2016(7):1847-1851. |
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| 作者姓名： | 鲁洲 周俊 何璟 |
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| 作者单位： | 华中农业大学农业微生物学国家重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金项目(31270136)，教育部留学回国人员科研启动基金项目([2009]1590)，教育部新世纪人才支持计划项目(NCET-08-0779) |
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| 摘 要： | 通过生物信息学分析,利用基因组发掘技术发现链霉菌SH-62基因组中至少含有37个次级代谢产物的生物合成基因簇,除了有4个基因簇分别与已知的肠道菌素、潮霉素A、尼日利亚菌素和格尔德霉素的生物合成基因簇具有高度同源性以外,其他基因簇的功能鲜见报道。在不同发酵培养基上培养链霉菌SH-62,利用高分辨率的LC-MS对发酵产物进行分析,发现链霉菌SH-62确实能够产生肠道菌素、潮霉素A和尼日利亚菌素,从而证明了基因组发掘策略确实能够指导代谢产物的分离及鉴定。
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| 关 键 词： | 链霉菌SH-62 基因组发掘 生物合成基因簇 天然产物 |
Separation and Characterization of Bioactive Natural Products in Streptomyces sp. SH-62 by Genome Mining |
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| Abstract: | There were at least 37 gene clusters for biosynthesis of secondary metabolites which were found in the genome of Streptomyces. sp. SH-62 by bioinformatics analyses. Besides 4 of them were highly homologous with the well-known biosyn-thetic gene clusters of enterocin, hygromycin A, nigericin and geldanamycin, respectively, biological functions of others had not been reported yet. Production of enterocin, hygromycin A and nigericin was detected by high-resolution LC-MS analyses of the Streptomyces. sp. SH-62 fermentation products in different media. It demonstrated that the genome mining strategy is feasible to direct detection and identification of metabolites. |
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| Keywords: | Streptomyces sp SH-62 genome mining biosynthetic gene cluster natural products |
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