木薯种质资源离体保存技术研究

为探讨木薯(Manihot esculenta Crantz)种质资源的离体保存方法,以GR891木薯品种试管苗为试材,研究了植物生长抑制剂(PP333、B9、S3307、ABA)对试管苗在常温(25℃)条件下的保存效果。结果表明,在培养室温度(25±2)℃、光照度1500~2 000 lx、光照时间12 h/d的条件下,培养基中加入PP333、B9和S3307均可提高木薯试管苗的存活率。在MS+6-BA 0.2 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+琼脂6.5 g/L培养基上,木薯常温保存的适宜PP333质量浓度为1.0~1.5 mg/L或B9和S3307质量浓度均为0.5~1.0 mg/L,保存240 d时存活率超过90%,试管苗根系发达、叶色浓绿、生长健壮,0.2~2.0 mg/L ABA培养基上试管苗长势和存活率都明显低于CK(对照)。

Study on in Vitro Conservation Technique of Cassava

The study aims to investigate the preservation methods of cassava germplasm in vitro.With material as cassava vari-eties GR891 cassava tissue culture plantlets,study on the plant growth retardants (PP333,B9,s3307,ABA) on in vitro preserva-tion at room temperature (25℃) temperature conditions. The results showed that in the culture room of temperature(25±2)℃, light intensity 1 500~2 000 lx and illumination time of 12 h/d,the survival rate of cassava test-tube plantlet conserved on medium with PP333,B9 and s3307 could be improved.On the medium of Ms+6-BA 0.2 mg/L+NAA 0.1 mg/L+ sucrose 30 g/L+agar 6.5 g/L,the optimum concentration of PP333 was between 1.0~1.5 mg/L,while B9 and s3307 both were 0.5~1.0 mg/L which would be suitable for inhibiting the growth of cassava plantlets,and improving the survival rate.After 240 d preservation, the average survival rate amounted to about 90%. Conservation plantlets in vitro have flourishing root system, much green leaf, robust growth. And 0.2~2.0 mg/L ABA medium, the growth and survival rate of plantlets were obviously lower than CK (contrast).