| 心包积液-肝坏死综合征高免卵黄抗体的研制 |
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| 引用本文： | 李新正,黄安群,陈理盾,姬向波,肖曙光,靳双星,黄炎坤. 心包积液-肝坏死综合征高免卵黄抗体的研制[J]. 湖北农业科学, 2016, 0(8). DOI: 10.14088/j.cnki.issn0439-8114.2016.08.033 |
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| 作者姓名： | 李新正 黄安群 陈理盾 姬向波 肖曙光 靳双星 黄炎坤 |
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| 作者单位： | 河南牧业经济学院动物科技学院,郑州,450046 |
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| 基金项目： | 河南省重点科技攻关项目(152102110089);河南牧业经济学院科技创新团队项目(HUAHE2015003) |
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| 摘 要： | 采取心包积液-肝坏死综合征病鸡的肝脏匀浆冻融,接种SPF鸡胚,制备尿囊液灭活水苗,用两种免疫程序对产蛋鸡进行免疫,用琼脂扩散试验检测其卵黄抗体水平,制备高免卵黄抗体。经中和试验和临床试验,结果显示高免卵黄抗体对该病的预防和治疗效果明显。
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| 关 键 词： | 心包积液-肝坏死综合征 组织灭活水苗 卵黄抗体 回归试验 中和试验 |
Development of High Immunity Yolk Antibody against Pericardial Effusion and Hepatic Necrosis Syndrome |
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| Abstract: | In this study, the liver homogenate from the sick chicken with pericardial effusion-hepatic necrosis syndrome was inoculated to sPF chicken embryo to prepare allantoic fluid inactivated vaccine after three times freezing and thawing. Two procedures were employed to immune the hens. Then, agar gel precipitation(AGP) was used to test the antibody level in yolk and prepared the yolk antibody. The neutralization test and clinical test identified that yolk antibody showed an obvious effect on preventing and curing for the disease. |
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| Keywords: | pericardial effusion-hepatic necrosis syndrome inactivated vaccine yolk antibody animal test neutralization test |
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