Effects of arbuscular mycorrhizal inoculation and phosphorus supply on the growth and nutrient uptake of Kandelia obovata (Sheue,Liu & Yong) seedlings in autoclaved soil |
| |
Institution: | 1. Department of Soil Science and Agricultural Chemistry, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India;2. Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur 721 302, India;3. Department of Plant and Soil Science, Texas Tech University, Lubbock, TX, USA;4. Agricultural and Ecological Research Unit, Indian Statistical Institute, Kolkata 700 108, India;5. Department of Agricultural Statistics, Uttar Banga Krishi Viswavidyalaya, Cooch Behar 736 165, India |
| |
Abstract: | This study evaluated the interactive effect of arbuscular mycorrhizal fungi (AMF) inoculation and exogenous phosphorus supply on soil phosphotases, plant growth, and nutrient uptake of Kandelia obovata (Sheue, Liu & Yong). We aimed to explore the ecophysiological function of AMF in mangrove wetland ecosystems, and to clarify the possible survival mechanism of mangrove species against nutrient deficiency. K. obovata seedlings with or without AMF inoculation (mixed mangrove AMF), were cultivated for six months in autoclaved sediment medium which was supplemented with KH2PO4 (0, 15, 30, 60, 120 mg kg?1). Then the plant growth, nitrogen and phosphorus content, root vitality, AMF colonization and soil phosphatase activity were analyzed. The inoculated AMF successfully infected K. obovata roots, developed intercellular hyphae, arbuscular (Arum-type), and vesicle structures. Arbuscular mycorrhizal fungi colonization ranged from 9.04 to 24.48%, with the highest value observed under 30 and 60 mg kg?1 P treatments. Soil P supply, in the form of KH2PO4, significantly promoted the height and biomass of K. obovata, enhanced root vitality and P uptake, while partially inhibiting soil acid (ACP) and alkaline phosphotase (ALP) activities. Without enhancing plant height, the biomass, root vitality and P uptake were further increased when inoculated with AMF, and the reduction on ACP and ALP activities were alleviated. Phosphorus supply resulted in the decrease of leaf N–P ratio in K. obovata, and AMF inoculation strengthened the reduction, thus alleviating P limitation in plant growth. Arbuscular mycorrhizal fungi inoculation and adequate P supply (30 mg kg?1 KH2PO4) enhanced root vitality, maintained soil ACP and ALP activities, increased plant N and P uptake, and resulted in greater biomass of K. obovata. Mutualistic symbiosis with AMF could explain the survival strategies of mangrove plants under a stressed environment (waterlogging and nutrient limitation) from a new perspective. |
| |
Keywords: | Arbuscular mycorrhizal fungi (AMF) Phosphorus Nutrient uptake Phosphatase |
本文献已被 ScienceDirect 等数据库收录! |
|