草莓离体再生体系的研究

目前植物转基因技术已成为植物定向遗传改良的主要手段,而建立稳定高频离体再生体系是实现遗传转化的基础和前提。笔者以3个主栽草莓品种丰香、弗吉尼亚、全明星为试材,综合研究影响草莓不定芽再生的各种因素,建立离体叶片高效再生系统。试验结果表明,对某一特定的品种来说,外植体基因型和外源激素种类及其合适浓度是决定再生的主要因子。建立草莓高频再生体系,TDZ是首选激素;某种激素随其组合激素不同而发挥功效不同;自然光照条件下培养不易发生玻璃化苗,而且苗分化好;AgNO3对弗吉尼亚叶片再生起抑制作用,但用含7 5mg/LAgNO3的继代培养基扩繁组培苗,却产生了大量的匍匐茎。

Study on Regeneration System for Strawberry in vitro

Nowadays, the gene transformation of plants has been one way of plant directional genetic breeding, and the establishment of high-frequency system is the base and precondition of genetic transformation. Three cultivars of strawberry, Toyonoka, Tudla and Allstar, were investigated for factors affecting the regeneration of adventitious bud, and a highly efficient regeneration system of leaf in vitro was established. The result showed that, to a specific genotype, the genotype of explants and the type of exogenous hormones were the main factors affecting regeneration. TDZ is the first choice hormone to establish the high frequency regeneration system of strawberry; Plant grew well under natural lightening, avoiding vitrification. In addition, AgNO3 inhibited leaf regeneration, but the generation of strawberry creeping stem is largely promoted after adding 7.5 mg/L AgNO_3 into the subculture medium.