| 绿僵菌不同菌株DNA 多态性的RAPD 分析 |
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| 引用本文： | 张立钦,林新春,毛胜凤.绿僵菌不同菌株DNA 多态性的RAPD 分析[J].浙江农林大学学报,2001,18(3):281-285. |
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| 作者姓名： | 张立钦 林新春 毛胜凤 |
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| 作者单位： | 1.浙江林学院 资源与环境系, 浙江 临安 311300 |
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| 基金项目： | 浙江省 151 人才 基金资助项目(浙人专1999-27) |
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| 摘 要： | 采用RAPD-PCR 技术分析了4 个绿僵菌菌株的DNA 遗传多态性。从160 条引物中筛选出27 条引物, 对各菌株进行PCR 扩增, 结果4 个绿僵菌菌株共扩增出334 个位点, 其中多态性位点299 个, 占89.5%, 表明各菌株间具有较丰富的遗传多态性。菌株DNA 多态性与原寄主和孢子形态表现出明显的相关性, 但与对松墨天牛幼虫的毒力间未表现出相关性。图2 表4 参7
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| 关 键 词： | 绿僵菌 随机扩增多态性DNA (RAPD) 分子标记 松墨天牛 |
| 收稿时间： | 2001-08-20 |
Analysis on isolates of Metarrhizium anisopliae by RAPD |
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| ZHANG Li-qin,LIN Xin-chun,MAO Sheng-feng.Analysis on isolates of Metarrhizium anisopliae by RAPD[J].Journal of Zhejiang A&F University,2001,18(3):281-285. |
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| Authors: | ZHANG Li-qin LIN Xin-chun MAO Sheng-feng |
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| Institution: | 1.Department of Resources and Environment, Zhejiang Forestry College, Lin,an, 311300, Zhejiang, China |
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| Abstract: | DNA of Metarrhizium anisopliae isolates was analyzed by RAPD. Twenty-seven primers screened and optimized out of 160 primers were applied to the proliferation of PCR. Three-hundred-four loci have been proliferated in 4 strains of M .anisopliae, 299 loci out of which are of polymorphism and account for 89.5%. All these suggest that each strain has an abundant genetic polyorphism. There is some correlation between DNA polymorphism of strains and their original hosts, size of conidiospore, distributions, but no obvious correlation between DNA polymorphism and virulence on Monochamus alternatus larvae. |
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