不同花生品种幼叶丛生芽诱导研究

为筛选高诱导率的花生品种并建立花生的高频再生体系,比较了22个花生品种在MS和1/2MS培养基中的萌发情况,同时以4d苗龄的花生幼叶为外植体,研究了花生幼叶丛生芽诱导率的品种差异性,以及羧苄青霉素(Carb)和头孢霉素(Cef)对花生丛生芽诱导的影响。结果表明:22个花生品种的种子在1/2MS培养基上萌发率均比在MS培养基上稍高。不同品种花生幼叶丛生芽诱导率差异明显,其中Hz006、Hz001、Hz012和Hz037幼叶外植体的丛生芽诱导率较高,均高于90%,Hz015最低,只有31.8%。添加500mg/L Carb对花生幼叶丛生芽分化无抑制作用,其中,Hz009添加500mg/L Carb后,丛生芽诱导率比不添加抗生素的CK提高13.5个百分点;Cef则对多数花生品种芽诱导率具有抑制作用,其中以Hz010最为明显,其丛生芽诱导率下降48.5个百分点。因此,花生种子预培养的适宜培养基为1/2MS,遗传转化时应选择Hz006、Hz001、Hz012和Hz037等高诱导率的花生品种,农杆菌介导花生转化时选择Carb抑菌比较合适。

Efficient Induction of Adventitious Buds from Peanut Leaflets of Various Varieties

In order to screen peanut genotypes with high induction efficiency of adventitious buds and to set up a high efficient regeneration system of peanut leaflets,MS and 1/2MS medium for peanut germination were compared based on the culture response of 22 different genotypes.The differences in induction frequency among various genotypes and the effects of Carb and Cef on the adventitious buds induction were also investigated using leaflets from 4 day-germinated peanut seeds as explants.It was shown that the germination rates of peanut seeds on 1/2MS medium were higher than those on MS medium.There were significant differences in bud induction rates among 22 genotypes.Moreover,the genotypes with induction rates above 90% were Hz006,Hz001,Hz012 and Hz037,while Hz015 had the lowest induction rate of 31.8%.No inhibition to bud induction was detected with 500 mg/L of Carb added to the medium and the induction rate of Hz009 was increased by 13.5 percentage points compared with that of CK.For most of the genotypes Cef had a negative effect on bud induction and the bud induction rate of Hz010 on the medium with Cef added was significantly dropped by 48.5 percentage points.It was concluded that 1/2MS was the suitable medium for peanut seeds germination and the genotypes of Hz006,Hz001,Hz012 and Hz037 with high induction rate were recommended for peanut genetic transformation.Additionally,Carb was a better antibiotic than Cef when they were used to control the over growth of agrobacteria on leaflet explants during the agrobacteria mediated transformation.