降低苹果梨组培过程中外植体褐化的研究

为克服苹果梨组织培养过程中外植体的褐变现象,提高试验材料的成活率,试验以苹果梨1 a生营养枝嫩梢为试材,通过不同培养基配方、不同药剂处理及水培处理,探讨降低苹果梨外植体褐变的影响因素.结果表明:1/3 MS培养基中的外植体成活率较高;液体培养基较固体培养基对降低外植体的褐变的效果好;水培处理可有效降低外植体的褐变率;生长季节取材,随外植体木质化程度的加深,随水培期的延长,外植体的褐变率呈现先降低后上升的趋势,水培4 d 处理的外植体成活率最高;最佳取材时期为盛花后期,将材料水培6 d,在1/3 MS 6-BA 1.0 mg/L IBA 0.2 mg/L Vc 75 mg/L中获得83.3%的外植体成活率.

Decreasing the Browning of Explants in the Process of Pingguoli Tissue Culture

To overcome the explants browning in tissue culture process of Pingguoli and improve the survival rate of test materials,this test adopted one year-old tender vegetative shoots of Pingguoli,set up several treatments of different media,different chemicals and water culture to explore the factors which reduce browning in plant of Pingguoli.The results showed that explants which in 1/3 MS medium had higher survival rate.Liquid medium had better effect than solid medium for decreasing browning rate of explants.Water culture treatment ould effectively reduce the explants browning rate.When the materials adopted in the growing season,as the explants lignification deepening and period of water culture extending,a tendency ould be found that the browning rate of explants rose at the beginning and dropped in the following.The explants had the highest survival rate with water culture for 4 days.Optimum material adopted period was anaphase of florescence,we ould got 83.3% survival rate on media 1/3 MS 6-BA 1.0 mg/L IBA 0.2 mg/L Vc 75 mg/L in which materials water culture for the 6 days.