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| TaPIMP1过量表达提高转基因小麦纹枯病抗性的研究 | |
| 引用本文： | 周淼平,周小青,张增艳,董娜,马鸿翔,姚金保. TaPIMP1过量表达提高转基因小麦纹枯病抗性的研究[J]. 核农学报, 2011, 25(3): 421-426,442 |
| 作者姓名： | 周淼平 周小青 张增艳 董娜 马鸿翔 姚金保 |
| 作者单位： | 1. 江苏省农业科学院生物技术所/江苏省农业生物学重点实验室,江苏南京,210014 2. 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室,北京,100081 3. 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室,北京100081;河南科技学院,河南新乡453003 |
| 基金项目： | 国家自然科学基金项目(30871523); 江苏省自然科学基金项目(BK2009322) |
| 摘 要： | 小麦纹枯病是由禾谷丝核菌和立枯丝核菌引起的世界性土传真菌病害,培育并推广小麦纹枯病抗病品种是控制该病害最经济和最有效的途径.本研究采用基因枪法将由Ubiquitin启动子驱动的TaPIMP1基因导入常规小麦品种扬麦158、扬麦12号和Alondra,获得转TaPIMP1基因小麦阳性植株17株;对上述转基因小麦T1和T2... |
| 关 键 词： | 小麦 转基因 TaPIMP1 纹枯病 |
| 收稿时间： | 2010-09-17 |
| 修稿时间： | 2010-12-13 |
OVER-EXPRESSION OF TaPIMP1 ENHANCED RESISTANCE TO WHEAT SHARP EYESPOT IN TRANSGENIC WHEAT |
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| ZHOU Miao-ping,ZHOU Xiao-qing,ZHANG Zeng-yan,DONG Na,MA Hong-xiang,Yao Jin-bao. OVER-EXPRESSION OF TaPIMP1 ENHANCED RESISTANCE TO WHEAT SHARP EYESPOT IN TRANSGENIC WHEAT[J]. Acta Agriculturae Nucleatae Sinica, 2011, 25(3): 421-426,442 | |
| Authors: | ZHOU Miao-ping ZHOU Xiao-qing ZHANG Zeng-yan DONG Na MA Hong-xiang Yao Jin-bao |
| Affiliation: | ZHOU Miao-ping1 ZHOU Xiao-qing1 ZHANG Zeng-yan2 DONG Na2,3 MA Hong-xiang1 Yao Jin-bao1 (1.Institute of Biotechnology/Provincial Key Laboratory of Agrobiology,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,2.National Key Science Facility of Crop Gene Resources and Gene Improvement/Key Laboratory of Crop Genetic and Breeding,Ministry of Agriculture/Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,3.Henan Institute of Science and Technology,Xinxiang,Henan 45... |
| Abstract: | Wheat sharp eyespot,mainly caused by Rhizoctonia cerealis Vander hoeven and Rhizoctonia solani Kuhn,is a soil-borne fungal disease worldwide.Developing and applying disease resistant varieties is the most effective and economical strategy to control the disease.TaPIMP1 gene controlled by maize Ubiquitin promoter was introduced into varieties of Yangmai 158,Yangmai 12 and Alondra via particle bombardment method,and 17 of transgenic plants were obtained.PCR analysis of TaPIMP1 gene in T1 and T2 transgenic pla... |
| Keywords: | wheat transformation TaPIMP1 sharp eyespot |
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