山羊黄体弥散性神经内分泌细胞的分布

为了探查山羊卵巢黄体中是否存在弥散性神经内分泌细胞,采用免疫组化链霉素抗生物素蛋白-过氧化物酶法对12~18月龄妊娠奶山羊卵巢中弥散性神经内分泌细胞标志物的分布进行了研究。结果显示:黄体组织中各区均含有催产素、神经元特异性烯醇化酶、S-100蛋白、突触素和5-羟色胺样免疫反应阳性细胞。这些细胞散在或成团分布,多呈圆形、卵圆形和三角形,一些细胞具有明显的突起。阳性细胞数由多至少顺序为:催产素、突触素、S-100蛋白、神经元特异性烯醇化酶、5-羟色胺。鉴于神经元特异性烯醇化酶、S-100蛋白、突触素和5-羟色胺是弥散性神经内分泌系统广谱的、共同的细胞标记物,结果提示山羊卵巢黄体中存在弥散性神经内分泌细胞。

Distribution of diffused neuroendorine system cells in the goat corpus luteum

Existence of diffused neuroendocrine system(DNES)cells in the corpus luteum of dairy goat has been determined by an immunohistochemistry streptavidin-peroxidase(SP)method. Using the SP method, the corpus luteum of 12-18-month-old dairy goats during pregnancy were studied and the following cell types were identified throughout the corpus luteum: oxytocin, neuron specific enolase, S-100 protein, synaptophysin, 5-hydroxytryptamine immunoreactive cells. The cells existed as individual or clustered. They were round, oval or triangle in shape. Some cells have obvious neuritis. The immunoreactive cells in one section in descending order were oxytocin, synaptophysin, S-100 protein, neuron specific enolase and 5-hydroxytryptamine. The existence of DNES cells was inferred from the presence of neuron specific enolase, S-100 protein, synaptophysin and 5-hydroxytryptamine which are the broad-spectrum markers for DNES.