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Potato cultivar identification using simple sequence repeats markers (SSR) |
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| Authors: | M. Moisan-Thiery S. Marhadour M. C. Kerlan N. Dessenne M. Perramant T. Gokelaere Y. Le Hingrat |
| Affiliation: | 1. Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche APBV Agrocampus Rennes, Fédération Nationale des Producteurs de Plant de Pommes de Terre (FNPPPT), Kera?ber, 29260, Ploudaniel, France 2. Unité Mixte de Recherche APBV Agrocampus Rennes, INRA, Kera?ber, 29260, Ploudaniel, France 3. Comité Nord Plants de Pommes de terre (CNPPT), Station ‘La Pigache’, Avenue Fran?ois Mitterrand, 62217, Beaurains, France 4. Roudouhir, Bretagne Plants, 29460, Hanvec, France 5. FNPPPT, siège social, 9 rue d’Athènes, 75009, Paris, France 6. FNPPPT Roudouhir, 29460, Hanvec, France |
| Abstract: | Summary Identification of potato cultivars is currently based on phenotypic characters. Crop inspections are needed at different stages and the increasing number of cultivars means the process is becoming more and more complex. Molecular markers are a possible complementary tool to identify potato cultivars and to rapidly check the identity of seeds lots. In this study 286 potato cultivars produced in France were characterized by Polymerase Chain Reaction (PCR) using Simple Sequence Repeats (SSR) markers. Sequential amplifications with 4 to 5 of the chosen SSR markers enabled complete discrimination between all the cultivars. The patterns were registered in a database and the procedure is now used routinely in France. |
| Keywords: | Genetic fingerprint microsatellites polymorphism Solarium tuberosum subsp.tuberosum |
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