新疆虫草人工发酵菌丝体食品安全性研究

目的]对新疆虫草人工发酵菌丝体进行毒理学研究。方法]以昆明种小白鼠和Wistar大白鼠为试验动物，用新疆虫草人工发酵菌丝体样品液对其进行灌胃或腹腔注射处理，以腹腔注射40 mg/kg环磷酰胺为阳性对照，不注射菌丝体样品液为阴性对照，研究该菌丝体对试验动物的急性经口毒性及对动物微核率和精子畸变率的影响。结果]新疆虫草人工发酵菌丝体对大、小鼠的急性经口LD-50均大于10g/kg；该虫草人工发酵菌丝体不同剂量处理组小白鼠嗜多染红细胞微核率、精子畸变率及大白鼠体重、食物利用率、血液生化指标与阴性对照组均无显著差异；病理组织学检查结果表明，各试验动物的脏器均未见与试验因素有关的病变。结论]新疆虫草人工发酵菌丝体对大、小白鼠未产生毒性作用。

Study on Food Safety of Artificial Fermentation Mycelia of Xinjiang Caterpillar Fungus

Objective] The aim was to study the toxicology of artificial fermentation mycelia of Xinjiang caterpillar fungus.Method] With Kunming mice(body weight of 18-22 g) and Wistar rats(body weight of 70-219 g) as the tested animals,they were given sample solution of artificial fermentation mycelia of Xinjiang caterpillar fungus by gavage or intraperitoneally injected with the sample solution,with the treatments of intraperitoneally injecting 40 mg/kg cyclophosphamide as positive CK and without intraperitoneal injection of sample solution of artificial fermentation mycelia of Xinjiang caterpillar fungus as negative CK,the acute oral toxicity and the effects of the mycelia on micronucleus rate and sperm abnormal rate of tested animals were studied.Result] The acute oral LD50 of artificial fermentation mycelia of Xinjiang caterpillar fungus on mice and rate were all bigger than 10 g/kg.The micronucleus rate of polychromatic erythrocytes(PCE) and sperm abnormal rate of mice and body weight,food utilization rate and blood biochemical indices of rats in treatments with artificial fermentation mycelia of Xinjiang caterpillar fungus at different doses had no significant differences with those of negative CK.The examination results of histopathology showed that the organs of tested animals had no lesions that related with experimental factors.Conclusion] The artificial fermentation mycelia of Xinjiang caterpillar fungus had no toxicity on rats and mice.