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| 利用AFLP分子标记鉴定苹果砧木 | |
| 引用本文： | 韩蕾,徐继忠,李振侠,孙叶红,张媛,邵建柱. 利用AFLP分子标记鉴定苹果砧木[J]. 华北农学报, 2008, 23(6) |
| 作者姓名： | 韩蕾 徐继忠 李振侠 孙叶红 张媛 邵建柱 |
| 作者单位： | 河北农业大学,园艺学院,河北,保定,071001;河北科技大学,生物科学与工程学院生物科学系,河北,石家庄,050018 |
| 基金项目： | 河北省科技厅资助项目(04220111D) |
| 摘 要： | 利用AFLP分子标记技术，对30个苹果砧木进行研究。从64对引物组合中筛选出3对多态性高、分辨能力强的引物用于扩增，共获得199条带，其中多态性带176条，多态性率为88．4％。扩增结果显示，3对引物组合在12个砧木中扩增出特征带，且每对引物组合均能将所有砧木鉴别开，表明AFLP技术用于苹果砧木鉴定的效率很高。通过聚类，对供试砧木的遗传关系进行分析，为中国优良苹果砧木资源的进一步收集和利用提供科学依据。 |
| 关 键 词： | 苹果砧木 鉴定 AFLP |
Identification of Apple Rootstocks with AFLP Molecular Markers |
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| HAN Lei,XU Ji-zhong,LI Zhen-xia,SUN Ye-hong,ZHANG Yuan,SHAO Jian-zhu. Identification of Apple Rootstocks with AFLP Molecular Markers[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(6) | |
| Authors: | HAN Lei XU Ji-zhong LI Zhen-xia SUN Ye-hong ZHANG Yuan SHAO Jian-zhu |
| Abstract: | Thirty apple rootstocks were subjected to AFLP analysis.Three primer combinations with high polymorphism and powerful distinctiveness selected from 64 primer combinations produced 199 fragments,of which 176 were polymorphic with a polymorphism percentage of 88.4%.The results showed that characteristic bands were amplified from 12 rootstocks,and any of the three primer combinations could identify all the rootstocks.AFLP was proved to be a very reliable method to identify apple rootstocks.The genetic relation... |
| Keywords: | Apple rootstock Identification AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |