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| 褪黑激素受体1a基因在内蒙古绒山羊垂体中的表达 | |
| 引用本文： | 郑竹清,石玲,王梦一,吴敬,刘志红,张燕军,苏蕊,赵艳红.褪黑激素受体1a基因在内蒙古绒山羊垂体中的表达[J].中国畜牧兽医,2012,39(4):39-41. |
| 作者姓名： | 郑竹清 石玲 王梦一 吴敬 刘志红 张燕军 苏蕊 赵艳红 |
| 作者单位： | 1. 内蒙古农业大学动物科学学院,内蒙古呼和浩特010018;内蒙古自治区动物遗传育种与繁殖重点实验室,内蒙古呼和浩特010018 2. 呼伦贝尔市环境科学研究所,内蒙古海拉尔,021008 3. 内蒙古农业大学食品工程与科学学院,内蒙古呼和浩特,010018 |
| 基金项目： | 内蒙古农业大学大学生科技创新基金,内蒙古农业大学博士启动基金(BJ09-2);国家自然基金(30760163,31160447);内蒙古自然基金(2009MS0402) |
| 摘 要： | 本试验从内蒙古绒山羊脑垂体中提取总RNA,根据已发表的相近物种褪黑激素受体1a(melatonin receptor 1 a,MTNR1a)基因序列设计引物,利用实时荧光定量逆转录多聚酶链反应(real-time quantitative,RT-PCR)技术、组织原位杂交技术检测MTNR1a基因在内蒙古绒山羊垂体中的表达。结果显示,通过RT-PCR方法检测到MTNR1a基因在内蒙古绒山羊垂体中表达,利用原位杂交技术进一步确定MTNR1a基因在垂体中表达分布。结果提示,垂体是褪黑激素作用的靶器官,褪黑激素对绒山羊季节性繁殖的影响有可能是与垂体分泌的某些激素相互作用而发挥作用的。 |
| 关 键 词： | MTNR1a 内蒙古绒山羊 RT-PCR 原位杂交 垂体 表达 |
| 收稿时间： | 2011-10-24 |
The Expression of MTNR1a Gene in Pituitary of Inner Mongolia Cashmere Goats |
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| ZHENG Zhu-qing , SHI Ling , WANG Meng-yi , WU Jing , LIU Zhi-hong , ZHANG Yan-jun , SU Rui , HAO Yan-hong.The Expression of MTNR1a Gene in Pituitary of Inner Mongolia Cashmere Goats[J].China Animal Husbandry & Veterinary Medicine,2012,39(4):39-41. | |
| Authors: | ZHENG Zhu-qing SHI Ling WANG Meng-yi WU Jing LIU Zhi-hong ZHANG Yan-jun SU Rui HAO Yan-hong |
| Institution: | 1. College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China;2. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction,Huhhot 010018, China;3. Hulunbeier Institute of Environmental Science, Hailaer 021008,China;4. College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018,China |
| Abstract: | In the study,the total RNA was extracted from pituitary of Inner Mongolia Cashmere goats.The primers were designed according to the published melatonin receptor gene(MTNR1a) in similar species.The expression of MTNR1a was detected by using RT-PCR and in situ hybridization in Inner Mongolia Cashmere goats.The results showed that the expression of MTNR1a was successfully detected by using RT-PCR,by using in situ hybridization futher defined the expression and distribution of MTNR1a.The results suggested that the pituitary is the target organ of the melatonin,the hormone secreted by pituitary may play an important role in the effect of melatonin on seasonal breeding of Cashmere goats. |
| Keywords: | MTNR1a Inner Mongolia Cashmere goats RT-PCR in situ hybridization pituitary expression |
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