外源Ca~(2+)对莲藕幼苗盐胁迫的缓解效应

以莲藕H_2(花藕品种,盐敏感)为材料,利用NaCl与Ca(NO_3)_2处理3片立叶的幼苗,研究外源Ca~(2+)对缓解植株盐胁迫的生理作用。结果显示:采用NaCl处理后,叶片中叶绿素、可溶性蛋白含量和SOD活性显著降低,而细胞膜透性、丙二醛和脯氨酸含量升高;利用不同浓度的Ca~(2+)进行缓解处理,发现外源施加低浓度的Ca~(2+)后,叶片叶绿素、可溶性蛋白含量和SOD活性均显著提高,脯氨酸含量较之前升高幅度更大,而细胞膜透性和丙二醛含量后期显著降低,说明Ca~(2+)对缓解莲藕盐胁迫具有较好的效果。当Ca~(2+)浓度增加时,缓解作用不明显,甚至出现抑制效果。

Alleviating Effects of Exogenous Ca2+ on Lotus Root Seedlings (Nelumbo nucifera Gaertn) under Salt Stress

Abstract: To search for alleviating effects of exogenous Ca2+ on lotus root seedlings under salt stress, theseedlings of lotus root H2(flower varieties of lotus root, salt sensitivity) with 3erect leaves were used as materials,treated by NaCl and Ca(NO3)2. The results showed that the content of soluble protein, leaf chlorophyll and SODactivity were significantly decreased, while the membrane permeability, MDA and proline content were increasedafter treatment with NaCl. After treated by different concentrations of Ca2+, the content of leaf chlorophyll, solubleprotein, proline and SOD activity were significantly enhanced when the exogenous Ca2+ at low concentration. Theproline content increased more greatly than before, while the cell membrane permeability and MDA content weredecreased significantly in later stage. In a total, low concentration of Ca2+ showed positively effect on alleviatingsalt stress, while high concentration of Ca2+ was negatively effect on lotus root.