红肉脐橙发育过程中抗氧化系统与活性氧的变化

 以红肉脐橙果肉为材料, 比较了不同时期谷胱甘肽(GSH) 、抗坏血酸(AsA) 含量及相关酶活性和活性氧(AOS) 产生速率的变化。结果表明: 果肉在整个发育过程中, 脱氢抗坏血酸还原酶(DHAR) 活性在很大程度上可以促进AsA积累, 两者呈显著的正相关( r = 0.870, P <0.05) , 并且与抗坏血酸过氧化物酶(AsA-POD) 活性在动态上彼此消长; 谷胱甘肽还原酶(GR) 的变化趋势与GSH相同,呈单峰型的变化, 两者的相关系数为r = 0.978 ( P < 0.01) ; AsA /DHAsA和GSH /GSSG比率在整个发育过程中呈下降的趋势, 而活性氧(H₂O₂ , O₂^·) 水平一直呈上升变化。

Changes in Antioxidant System and Active Oxygen Species in Flesh of Cara Cara (Citrus sinenisis) during Development

Cara Cara 〔Citrus sinenisis (L. ) Osbeck ‘Cara Cara’〕was used to study the contents of glutathione (GSH) , ascorbate (AsA) , active oxygen species (AOS) and changes in the activities of correlative enzymes during development. The results showed that the contents of AsA accumulated with increase inthe activity of dehydroascorbate reductase (DHAR) during the fruit development and the correlation coefficient was 0.870, P < 0.05. The activity of DHAR showed opposite trend with ascorbate peroxidase (AsA-POD).The changes in glutathione reductase (GR) activities and GSH were similar to display a single-peak pattern and their correlation coefficient was 0.978 ( P < 0.01). The ratios of AsA /DHAsA and GSH /GSSG both descended, while the contents of AOS increased throughout fruit development.