黄瓜嫩果皮色与色素含量的关系

试材为不同皮色的8个黄瓜品种和以其为亲本配制的10个组合。将上述材料设区种植,每小区30株。先按果色差异对照色谱目测分类,再随机抽样测定色素含量。雌花开花至商品瓜成熟(约7～10 d)取样,沿果实纵向削下宽1 cm,厚0.2cm左右的表皮,用打孔器(直径0.56 cm)取圆片20片,在80％的丙酮液中遮光浸提24 h,用721型分光光度计比色。

Correlations of Immature Skin Color and Pigments in Cucumber

AIM:To investigates the role of phosphatidic acid (PA) in the expression of several inflammatory mediators produced by glomerural macrophages (GMΦ).METHODS:The study was performed on a rat model of accelerated anti-glomerural basement membrane (anti-GBM) glomerulonephritis (GN). GN-GMΦ were isolated and identified. Peritoneal MΦ (P-MΦ) of both normal and GN rats were used as controls. Block and reverse test were investigated with rhIL-1β stimulated, lisofylline (LSF) and phosphatidic acid (PA). Macrophage expression of ICAM-1 and TGF-β₁ were assessed at the level of protein and gene by immunocytochemistry, northern blot and RT-PCR.RESULTS:(1) After stimulated with rhIL-1β, GN-GMΦ produced much more ICAM-1, MCP-1 and TGF-β₁ than P-MΦ, and it's gene expression was similar as protein product. (2) mRNA expression of these factors was up-regulated again after the GN-GMΦ were pretreated with LSF then PA was added.CONCLUSIONS:Since GN-GMФ plays an important role for PA in the mediation of glomerular injury, inhibiting of PA production is the keypoint of blocking MФ mediated inflammatory effects. LSF may be an effective medicine in therapy for acute inflammatory forms of GN.