Armillaria species on tea in Kenya identified using isozyme and DNA sequence comparisons |
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| Authors: | E Mwenje B D Wingfield M P A Coetzee H Nemato M J Wingfield |
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| Institution: | Department of Applied Biology and Biochemistry, National University of Science and Technology, PO Box AC 939, Ascot, Bulawayo, Zimbabwe;and;Department of Genetics, Tree Protection Co-operative Programme (TPCP), Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa |
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| Abstract: | The aim of this study was to identify seven Armillaria isolates obtained from diseased tea bushes in Kenya using pectic enzyme profiles, PCR-RFLP and IGS-I DNA sequence data. The combination of these identification methods confirmed the presence of three distinct Armillaria groups. One of these groups resembled Zimbabwean group I ( A. fuscipes ). The second group was phylogenetically closely related to A. mellea ssp. nipponica . The third group was different from all other African isolates examined, but had isozyme patterns, especially of pectin methylesterases (PMEs), similar to those of isolates related to A. mellea ssp. nipponica. Analyses of sequence data suggested that this group is phylogenetically closely related to A. hinnulea from Australia and New Zealand. |
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| Keywords: | armillaria root rot Camellia sinensis IGS-1 rRNA isozymes taxonomy |
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