| Dynamic changes of telomeric restriction fragment (TRF) lengths in cells during the developmental process from embryos to seedlings and a comparison with the embryonal calli in Ginkgo biloba L. |
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| 作者姓名： | Liu Di Zhang Xiao-mei Hua Xin Qiao Nan Song Han Lu Hai Guo Hui-hong Li Feng-lan |
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| 作者单位： | Liu Di Zhang Xiao-mei Hua Xin Qiao Nan Song Han Lu Hai Guo Hui-hong Li Feng-lan* College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P. R. China |
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| 摘 要： | Telomeres are the structures that locate at the terminals of linear eukaryotic chromosomes. They can play essential roles in many cellular processes. The terminal location of Arabidopsis-type TTTAGGG tandem repeats were thought to be highly conserved. The terminal location of Ginkgo biloba L. consisting of TTTAGGG tandem repeats, were confirmed by Bal31 exonuclease degradation and Southern blotting. By comparing telomeric restriction fragment (TRF) lengths at different developmental stages from embryos to seedlings, a fluctuant tendency towards variation was found in these samples. The TRF length of embryos was also compared with that of embryonal calli and an upward trend was discovered in callus culture. The results suggest that there should be a telomerase mechanism or/and ALT mechanism for the maintenance of telomere length.
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| 关 键 词： | 银杏树 维护方法 栽培方法 种子 |
| 收稿时间： | 22 October 2006 |
| 修稿时间： | 2006-10-22 |
Dynamic changes of telomeric restriction fragment (TRF) lengths in cells during the developmental process from embryos to seedlings and a comparison with the embryonal calli in <Emphasis Type="Italic">Ginkgo biloba</Emphasis> L. |
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| Liu Di Zhang Xiao-mei Hua Xin Qiao Nan Song Han Lu Hai Guo Hui-hong Li Feng-lan.Dynamic changes of telomeric restriction fragment (TRF) lengths in cells during the developmental process from embryos to seedlings and a comparison with the embryonal calli in Ginkgo biloba L.[J].Forestry Studies in China,2007,9(2):127-131. |
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| Authors: | Liu Di Zhang Xiao-mei Hua Xin Qiao Nan Song Han Lu Hai Guo Hui-hong Li Feng-lan |
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| Institution: | College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China |
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| Abstract: | Telomeres are the structures that locate at the terminals of linear eukaryotic chromosomes. They can play essential roles in many cellular processes. The terminal location of Arabidopsis-type TTTAGGG tandem repeats were thought to be highly conserved. The terminal location of Ginkgo biloba L. consisting of TTTAGGG tandem repeats, were confirmed by Bal31 exonuclease degradation and Southern blotting. By comparing telomeric restriction fragment (TRF) lengths at different developmental stages from embryos to seedlings, a fluctuant tendency towards variation was found in these samples. The TRF length of embryos was also compared with that of embryonal calli and an upward trend was discovered in callus culture. The results suggest that there should be a telomerase mechanism or/and ALT mechanism for the maintenance of telomere length. |
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| Keywords: | Ginkgo biloba L maintenance mechanism telometic restriction fragment length |
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