| 匈牙利速生型刺槐遗传转化再生体系的建立 |
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| 引用本文： | qaiguang@.com。.匈牙利速生型刺槐遗传转化再生体系的建立[J].中国农学通报,2006,22(5):128-128. |
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| 作者姓名： | qaiguang@.com。 |
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| 作者单位： | 北京林业大学生物科学与技术学院,北京,100083 |
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| 基金项目： | 国家林业局"948"项目;北京林业大学校科研和教改项目 |
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| 摘 要： | （北京林业大学生物科学与技术学院，北京 100083）
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| 关 键 词： | 中间砧 中间砧 叶片 矿质营养 含量 年动态变化 |
| 收稿时间： | 2006-02-27 |
| 修稿时间： | 2006-02-272006-03-01 |
Establishment of a Genetic Transformation System in Fast Growing Robinia pseudocacia from Hungary |
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| Q.Establishment of a Genetic Transformation System in Fast Growing Robinia pseudocacia from Hungary[J].Chinese Agricultural Science Bulletin,2006,22(5):128-128. |
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| Authors: | Q |
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| Institution: | College of Biologiocal Sciences and Biotechnology, Beijing Forestry University, Beijing 100083 |
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| Abstract: | Adventitious shoots were induced from the raches of compound leaves of fast growing variety of Robinia pseudocacia introduced from Hungary in this study. The optimized medium for raches explants contains MS base medium combined with 6-BA 1.0 mg/L and NAA 0.5 mg/L, shoot regeneration rate is 77.5%. The medium for rooting of shoots is optimized as I/2MS base medium supplemented with NAA 0.2mg/L and IBA 0.1mg/L, with a rooting efficiency of 100%. Hygromycin at 10mg/L in the medium gives effective inhibition to the regeneration of shoots from the explants; in addition, hygromycin at 5mg/L in the medium shows obvious inhibition to the regeneration of roots from the shoots. |
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| Keywords: | Robinia pseudocacia L Explant Regeneration system |
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