| 高效液相色谱法测定调制乳中低聚果糖的含量 |
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| 引用本文： | 万鹏,赵贞,刘春霞,岳虹,李翠枝,邵建波.高效液相色谱法测定调制乳中低聚果糖的含量[J].乳业科学与技术,2015,38(3):23-25. |
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| 作者姓名： | 万鹏 赵贞 刘春霞 岳虹 李翠枝 邵建波 |
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| 作者单位： | 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特,010110 |
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| 基金项目： | “十二五”国家科技部支撑计划项目(2012BAK17B00) |
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| 摘 要： | 建立调制乳中低聚果糖高效液相色谱的测定方法.采用水作为提取剂,乙腈沉淀蛋白,氨基色谱柱分离,流动相为70％乙腈溶液,示差检测器检测.每100 g调制乳中低聚果糖添加量为20～500 mg时,回收率为80.2％～107.1％,定量检出限为20 mg/100g.该方法操作简便、检测准确,可用于调制乳中低聚果糖的检测.
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| 关 键 词： | 高效液相色谱 低聚果糖 调制乳 |
High Performance Liquid Chromatography Determination of Fructooligosaccharides in Modified Milk |
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| WAN Peng,ZHAO Zhen,LIU Chunxia,YUE Hong,LI Cuizhi,SHAO Jianbo.High Performance Liquid Chromatography Determination of Fructooligosaccharides in Modified Milk[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2015,38(3):23-25. |
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| Authors: | WAN Peng ZHAO Zhen LIU Chunxia YUE Hong LI Cuizhi SHAO Jianbo |
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| Institution: | (Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010110, China) |
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| Abstract: | A method for the determination of fructooligosaccharides in modified milk by high performance liquid
chromatography (HPLC) was developed. Fructooligosaccharides were extracted with water. The analysis was performed on
a NH2 column using 70% acetonitrile in water as the mobile phase. The recoveries of fructooligosaccharides at four spiked
levels between 20 and 500 mg/100 g ranged from 80.2% to 107.1%. The limit of quantification was 20 mg/100 g. This
method is accurate, simple and suitable for the analysis of fructooligosaccharides in modified milk. |
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| Keywords: | high performance liquid chromatography (HPLC) fructooligosaccharides modified milk |
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