抑制素基因免疫黄牛的黄体发育与免疫纯度及剂量的关系

为探讨双拷贝抑制素pcISI基因疫苗纯度和剂量对肉牛黄体发育的影响,将150头同期发情的南阳黄牛随机分为8个试验组和2个对照组,试验组用pcISI按2种纯度、4种剂量(0.75、1.50、2.25、3.00 mg/头)进行免疫处理,2个对照组分别用空质粒和生理盐水进行处理;初次免疫21 d后进行加强免疫。结果表明:无论用高纯度还是低纯度疫苗免疫,2.25 mg/头剂量处理对黄体的作用效果最明显,且抗体水平与黄体发育密切相关,2.25 mg/头抑制素融合表达质粒组的黄体显著大于0.75 mg/头组(P<0.05),抗体阳性牛两侧黄体显著比抗体阴性牛的大(P<0.05)。

Effect of purity and dose of inhibin gene vaccine on corpus luteum development of beef cattle

Estrus synchronized yellow cattle(n=150) were divided into 8 experimental groups immunized with different dosages(0.75,1.50,2.25,3.00 mg per animal) of highly-or lowly-purified inhibin fusion expressing plasmid pcISI and 2 control groups immunized with plasmid pcISI or physiological saline to investigate the effect of purity and dosage on corpus luteum development of yellow cattle immunized with inhibin gene expressing plasmid pcISI.Booster immunization was done 21 d after the primary immunization.The results showed that regardless of the highly-or lowly-purified plasmid immunization.inhibin fusion expressing plasmid in dosage of 2.25 mg per animal showed the best effect on corpus luteum size,which in experimental group with dosage of 2.25 mg per animal was remarkably larger than that in experimental group with dosage of 0.75 mg per animal(P<0.05).Inhibin antibody level is closely related to the development of corpus luteum.Corpus luteum sizes on both sides in inhibin antibody-positive cattle were bigger than those in inhibin antibody-negative ones(P<0.05).