| 规模化猪场脐带血猪瘟病原检测及遗传变异分析 |
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| 引用本文： | 戴爱玲,刘建奎,王德永,魏春华,林日丹,杨小燕.规模化猪场脐带血猪瘟病原检测及遗传变异分析[J].中国兽医杂志,2019(3):17-20,25,I0002. |
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| 作者姓名： | 戴爱玲 刘建奎 王德永 魏春华 林日丹 杨小燕 |
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| 作者单位： | 1.龙岩学院生命科学学院;2.福建省生猪疫病防控工程技术研究中心 |
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| 基金项目： | 福建省科技计划引导性项目(2017N0022) |
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| 摘 要： | 为了了解某规模化猪场母猪猪瘟病毒(CSFV)带毒及病原遗传变异情况,应用荧光定量PCR对117份分娩母猪的脐带血进行猪瘟病原检测,对阳性样品进行CSFV E2基因RT-PCR扩增,研究CSFV遗传变异特性。结果表明,该猪场猪瘟病原阳性率为7.69%(9/117),5株CSFVE2基因之间的核苷酸同源性为97.8%~100%,与参考株的同源性为80.0%~97.5%,与C株和HCLV株的同源性分别为95.6%~96.7%、96.4%~97.5%;5株CSFVE2基因推导的氨基酸同源性为97.8%~100%,与参考株之间同源性为83.5%~96.7%,与C株和HCLV株同源性均为95.5%~96.7%。遗传进化树分析表明,5株CSFV E2基因属于1.1亚群,与疫苗株HCLV亲缘性最近;氨基酸变异位点分析表明,5株CSFV E2基因在位于抗原决定区内氨基酸发生较小程度的变异。表明该猪场通过胎盘屏障感染仔猪的猪瘟病毒可能来源疫苗株,这种现象应该引起重视。
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| 关 键 词： | 脐带血 猪瘟 E2基因 序列分析 |
Detection and genetic variation analysi of classical swine fever virus from umbilical cord blood in large-scale pig farms |
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| DAI Ai-ling,LIU Jian-kui,WANG De-yong,WEI Chun-hua,LIN Ri-dan,YANG Xiao-yan.Detection and genetic variation analysi of classical swine fever virus from umbilical cord blood in large-scale pig farms[J].Chinese Journal of Veterinary Medicine,2019(3):17-20,25,I0002. |
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| Authors: | DAI Ai-ling LIU Jian-kui WANG De-yong WEI Chun-hua LIN Ri-dan YANG Xiao-yan |
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| Institution: | (College of Life Sciences ,Longyan University ,Longyan 364012 ,China;Fujian Engineering Research Center for Swine Disease Control and Prevention ,Longyan 364012 ,China) |
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| Abstract: | DAI Ai-ling;LIU Jian-kui;WANG De-yong;WEI Chun-hua;LIN Ri-dan;YANG Xiao-yan(College of Life Sciences ,Longyan University ,Longyan 364012 ,China;Fujian Engineering Research Center for Swine Disease Control and Prevention ,Longyan 364012 ,China) |
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| Keywords: | umbilical cord blood classical swine fever virus E2 gene sequential analysis |
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