| 驴源马流产沙门菌的耐药表型及毒力基因检测 |
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| 引用本文： | 孙莹慧,刘康军,陶璐瑶,孟霞,崔璐莹,姜桂苗,赵付伟,李建基,王亨. 驴源马流产沙门菌的耐药表型及毒力基因检测[J]. 中国兽医学报, 2019, 0(10): 1959-1962 |
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| 作者姓名： | 孙莹慧 刘康军 陶璐瑶 孟霞 崔璐莹 姜桂苗 赵付伟 李建基 王亨 |
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| 作者单位： | 1.扬州大学兽医学院;2.江苏省动物重要疫病与人兽共患病防控协同创新中心;3.东阿阿胶股份有限公司 |
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| 基金项目： | 扬州大学专业学位研究生教学案例库建设资助项目;扬州大学教学改革研究资助项目(YZUJX2017-13B,YZUJX2016-19B);扬州大学优秀青年骨干教师资助项目;江苏高校优势学科建设工程资助项目(PAPD);江苏高校品牌专业建设工程资助项目(TAPP) |
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| 摘 要： | 为了解驴源马流产沙门菌临床分离株的耐药表型及毒力基因的携带情况,本试验通过K-B药敏纸片扩散法对9株马流产沙门菌进行11种临床常用抗菌药物的敏感性测试,采用PCR方法检测与沙门菌致病性相关的10种毒力基因。结果显示:9株驴源马流产沙门菌存在2种耐药表型,对链霉素耐药率达100%,对阿莫西林耐药率为33%;10种毒力基因中仅traT未被检出,invA、avrA、ssaQ、mgtC、sopB、spvC、spvR、pefA和misL的检出率均为100%。结果表明,驴源马流产沙门菌耐药谱较为单一,但携带的毒力基因为多样性组合。
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| 关 键 词： | 驴 马流产沙门菌 耐药性 毒力基因 |
Detection of antimicrobial resistance and virulence genes of Salmonella abortusequi isolated from donkeys |
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| SUN Ying-hui,LIU Kang-jun,TAO Lu-yao,MENG Xia,CUI Lu-ying,Jiang Gui-miao,ZHAO Fu-wei,LI Jian-ji,WANG Heng. Detection of antimicrobial resistance and virulence genes of Salmonella abortusequi isolated from donkeys[J]. Chinese Journal of Veterinary Science, 2019, 0(10): 1959-1962 |
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| Authors: | SUN Ying-hui LIU Kang-jun TAO Lu-yao MENG Xia CUI Lu-ying Jiang Gui-miao ZHAO Fu-wei LI Jian-ji WANG Heng |
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| Affiliation: | (College of Veterinary Medicine ,Yangzhou University ,Yangzhou , Jiangsu 225009 , China;Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses.Yangzhou,Jiangsu 225009, China;Dong-E-E-Jiao Co. ,Ltd ,Liaocheng ,Shandong 252201) |
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| Abstract: | SUN Ying-hui;LIU Kang-jun;TAO Lu-yao;MENG Xia;CUI Lu-ying;Jiang Gui-miao;ZHAO Fu-wei;LI Jian-ji;WANG Heng(College of Veterinary Medicine ,Yangzhou University ,Yangzhou , Jiangsu 225009 , China;Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses.Yangzhou,Jiangsu 225009, China;Dong-E-E-Jiao Co. ,Ltd ,Liaocheng ,Shandong 252201) |
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| Keywords: | donkey Salmonella abortusequi antimicrobial resistance virulence gene |
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