|
|||||
|
|
| 草鱼GSTR基因外显子1、外显子2的SNPs筛选及其与生长性状的关联分析 | |
| 引用本文： | 刘小献,白俊杰,徐 磊,于凌云,樊佳佳,韩林强.草鱼GSTR基因外显子1、外显子2的SNPs筛选及其与生长性状的关联分析[J].华中农业大学学报,2011,30(6):753-758. |
| 作者姓名： | 刘小献 白俊杰 徐 磊 于凌云 樊佳佳 韩林强 |
| 作者单位： | 1. 中国水产科学研究院珠江水产研究所/中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广州 510380;大连海洋大学生命科学与技术学院,大连 116023 2. 中国水产科学研究院珠江水产研究所/中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广州,510380 3. 上海海洋大学水产与生命学院,上海,201306 |
| 基金项目： | 现代农业产业技术体系建设专项(Nycytx-49-07) |
| 摘 要： | 根据草鱼EST数据库中的rho型谷胱甘肽硫转移酶基因cDNA序列设计引物,扩增预计存在的SNP位点.采用PCR产物测序法和PCR-RFLP法,在珠江水系草鱼养殖群体中筛选到2个SNP位点,分别位于外显子1和外显子2上,为C-T突变,且属于同义突变,统计了多态位点在群体中的分布:C十129T位点CC型占0.69％,TC型... |
| 关 键 词： | 草鱼 SNPs 谷胱甘肽硫转移酶 rho型谷胱甘肽硫转移酶基因 多态性 生长 |
| 收稿时间： | 2011/3/29 0:00:00 |
SNPs in exon 1,exon 2 of GSTR gene and its relationship with growth traits in grass carp |
|
| LIU Xiao-xian,BAI Jun-jie,XU Lei,YU Ling-yun,FAN Jia-jia,HAN Lin-qiang.SNPs in exon 1,exon 2 of GSTR gene and its relationship with growth traits in grass carp[J].Journal of Huazhong Agricultural University,2011,30(6):753-758. | |
| Authors: | LIU Xiao-xian BAI Jun-jie XU Lei YU Ling-yun FAN Jia-jia HAN Lin-qiang |
| Institution: | LIU Xiao-xian1,2 BAI Jun-jie1 XU Lei3 YU Ling-yun1 FAN Jia-jia1 HAN Lin-qiang3 1.Key Laboratory of Tropical and Subtropical Fish Breeding & Cultivation of CAFS/Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China,2.College of Life Science and Technology,Dalian Ocean University,Dalian 116023,3.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306 |
| Abstract: | Primers were designed according to glutathione S-transferase-rho gene from EST database of grass carp.The PCR products were sequenced for detecting polymorphisms in cultured population and two SNPs sites were found:C+129T in exon 1 and C+192T in exon 2.Both SNPs are synonymous mutation.The polymorphisms were genotyped by PCR-RFLP methods.The percentage of CC,TC and TT genotype in C+129T site was 0.69%,18.75% and 80.56%,respectively.The percentage of CC,TC,and TT genotype in C+192T site was 25.4%,48.6% and 2... |
| Keywords: | grass carp SNPs gluthione S-transferase GSTR polymorphisms growth |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《华中农业大学学报》浏览原始摘要信息 | |
| 点击此处可从《华中农业大学学报》下载免费的PDF全文 | |