辣蓼黄酮中芦丁、槲皮素与槲皮苷的含量分析

前期研究中运用大孔吸附树脂法对辣蓼黄酮进行分离纯化并对其抗氧化活性进行初步试验,获得令人满意的结果。为了获得质量稳定的辣蓼黄酮,为工业化生产中的质量控制提供实验依据,通过高效液相色谱法,以芦丁、槲皮素与槲皮苷为对照品对辣蓼总黄酮进行目标成分含量测定。结果表明,运用高效液相色谱法测定辣蓼黄酮乙酸乙酯部分(FEA)中芦丁、槲皮素与槲皮苷的含量分别为281.97、160.98、83.31 mg/g,辣蓼黄酮正丁醇部分(FNB)中芦丁、槲皮素与槲皮苷的含量分别为150.33、147.51、73.82 mg/g。该方法简单、准确、灵敏,精密度、重复性好,可作为辣蓼黄酮中芦丁、槲皮素与槲皮苷含量分析的参考方法。

Determination of contents of rutin, quercetin and quercitrin in flavonoids from Polygonum hydropiper

The flavonoids of Polygonum hydropiper were separatedSand purified by means of macroporous adsorption resin, and its antioxidant activity was tested in previous research, which gained satisfied results. In order to obtain flavonoids of Polygonum hydropipe with stable quality, and provide evidence for quality control in industrial production, high performance liquid chromatography for determination of total flavonoids in polygonum hydropiper was used with rutin, quercetin and quercitrin asSreference substance. The results showed that the contents of rutin, quercetin and quercitrin from flavonoids ethyl acetate (FEA) in Polygonum hydropipe were 281.97, 160.98, 83.31 mg/g respectively; The contents of rutin, quercetin and quercitrin of flavonoids n-butanol (FNB) in polygonum hydropiper were 150.33, 147.51, 73.82 mg/g respectively. The method demonstrated advantage of simply,accuraty and sensitivity, and successfully applied to the analysis of rutin, quercetin and quercitrin content in flavonoids from Polygonum hydropipe.