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ISSR Analysis of 26 General Species in Gladiolus hybridus Hort |
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| Authors: | WANG Jingang GUO Ying CHE Daidi LIU Shenkui YANG Chuanpin College of Horticulture Northeast Agricultural University Harbin China Key Laboratory of Heredity Breeding Northeast Forestry University Harbin China Departement of Foreign Language Harbin Normal University Harbin China |
| Institution: | [1]College of Horticulture, Northeast Agricultural University, Harbin 150030, China [2]Key Laboratory of Heredity Breeding, Northeast Forestry University, Harbin 150040, China [3]Departement of Foreign Language, Harbin Normal University, Harbin 150080, China |
| Abstract: | ISSR analysis is applied in this study to research the classification and genetic relationship of 26 cultivars of G. hybridus Hort. Total 19 arbitrary primers screened from 100 primers were used for further PCR and diversity analysis. A total of 110 ISSR sites were detected with a mean of 5.63 fragments amplified for each primer. A total of 103 polymorphic DNA fragments were detected among all the 110 amplified fragments, which accounted for a high level of 93.6% of all and could be used to identify different cultivars. The results revealed that the idioplasm resource of G. hybridus Hort cutting flower cultivar had a narrow genetic basis on molecular level, and certain genetic relationship existed in summer large-flowers of G. hybridus Hort varieties. |
| Keywords: | Gladiolus hybridus Hort ISSR polymorphism |
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