| 香蕉延长因子1A（MaEF1A）基因的分离及特征分析 |
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| 引用本文： | 刘菊华,徐碧玉,陈敦忠,张静,张建斌,贾彩红,金志强.香蕉延长因子1A（MaEF1A）基因的分离及特征分析[J].果树学报,2011(5):825-830. |
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| 作者姓名： | 刘菊华 徐碧玉 陈敦忠 张静 张建斌 贾彩红 金志强 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所/农业部热带生物技术重点开放实验室;中国热带农业科学院海口试验站; |
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| 基金项目： | 现代农业产业技术体系建设专项(nycytx-33); 公益性行业(农业)科研专项(nyhyzx07-029); 热带生物技术研究所中央级公益性科研院所基本科研业务费专项(ITBB110202) |
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| 摘 要： | 采用筛选香蕉果实采后cDNA文库的方法,首次获得了一条长为1 341 bp的cDNA序列,命名为MaEF1A。生物信息学分析结果表明,它与麝香百合延长因子1A 3的mRNA有84%的同源性,与烟草延长因子1A的mRNA有83%的同源性,推测它可能为编码香蕉延长因子1A（elongation factor-1 alpha...
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| 关 键 词： | 香蕉 cDNA文库 MaEF1A RT-PCR |
Isolation and functional analysis of a elongation factor 1A(MaEF1A) gene from banana |
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| LIU Ju-hua,XU Bi-yu,CHEN Dun-zhong,ZHANG Jing,ZHANG Jian-bin,JIA Cai-hong,JIN Zhi-qiang.Isolation and functional analysis of a elongation factor 1A(MaEF1A) gene from banana[J].Journal of Fruit Science,2011(5):825-830. |
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| Authors: | LIU Ju-hua XU Bi-yu CHEN Dun-zhong ZHANG Jing ZHANG Jian-bin JIA Cai-hong JIN Zhi-qiang |
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| Institution: | LIU Ju-hua1,XU Bi-yu1,CHEN Dun-zhong1,ZHANG Jing1,ZHANG Jian-bin1,JIA Cai-hong1,JIN Zhi-qiang1,2(1Key laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101 China,2Haikou Experimental Station,Haikou 570102 China) |
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| Abstract: | A cDNA sequence with length of 1 341 bp named MaEF1A had been obtained by screening postharvest banana fruit cDNA library.The results of bioinformatics analysis showed that MaEF1A shared 84% and 83% homologous with Lilium longiflorum Thumb and tobacco,respectively,which was suggested to be a banana elongation factor 1 alhpa(EF 1A).MaEF1A contained an integrated ORF and three conserved regions of GTP-EFTU,GTP-EFTU-02 and GTP-EFTU-03.The expressions of MaEF1A in different banana tissues and fruit in different... |
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| Keywords: | Banana Fruit cDNA library MaEF1A RT-PCR |
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