| 采用Fluo-3 AM-ester探针研究桃不同组织的Ca^2＋信号分布 |
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| 引用本文： | 鲁振华,王志强,彭斌,牛良,崔国朝,刘淑娥.采用Fluo-3 AM-ester探针研究桃不同组织的Ca^2＋信号分布[J].果树学报,2011(5):915-917,I0001. |
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| 作者姓名： | 鲁振华 王志强 彭斌 牛良 崔国朝 刘淑娥 |
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| 作者单位： | 中国农业科学院郑州果树研究所国家桃葡萄改良中心; |
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| 基金项目： | 公益性行业(农业)科研专项(200903044) |
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| 摘 要： | 钙作为植物最重要的矿质元素之一,在植物生理和生化活动中起重要作用。采用激光扫描共聚焦显微镜,以Fluo-3 AM-ester为荧光探针研究了桃果肉和叶柄组织中Ca2＋的分布,结果表明Fluo-3 AM-ester可作为荧光探针对桃不同组织中游离的Ca2＋信号强度和分布特征进行分析。不同的物镜观察（10×和20×）下,C...
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| 关 键 词： | 桃 Ca2+信号 激光扫描共聚焦显微镜 Fluo-3AM-ester |
Using Fluo-3 AM-ester Probe as indicator for studying calcium distribution in different tissues of peach |
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| LU Zhen-hua,WANG Zhi-qiang,PENG Bin,NIU Liang,CUI Guo-chao,LIU Shu-e.Using Fluo-3 AM-ester Probe as indicator for studying calcium distribution in different tissues of peach[J].Journal of Fruit Science,2011(5):915-917,I0001. |
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| Authors: | LU Zhen-hua WANG Zhi-qiang PENG Bin NIU Liang CUI Guo-chao LIU Shu-e |
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| Institution: | LU Zhen-hua,WANG Zhi-qiang,PENG Bin,NIU Liang,CUI Guo-chao,LIU Shu-e(Zhengzhou Fruit Research Institute,CAAS,National Peach and Grape Improvement Center,Zhengzhou,Henan 450009 China) |
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| Abstract: | Calcium as one of the essential plant nutrients plays a key role in plant physiology and biochemistry activities.Fluo-3 AM-ester was used as an indicator to detect the calcium ion signal characteristics in fruit and petiole of peach with Laser Scanning Confocal Microscope(LSCM).The result showed that Fluo-3 AM-ester could act as the indicator to visualize the intensity and its distribution of Ca2+ Fluorescent signal.No significant difference in intensity was observed with 10× and 20× objectives.The signal was detected mainly in vascular bundle in petiole,but in cell wall in flesh.The intensity was stronger in petiole than in flesh,contributing to its functional roles in different tissues. |
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| Keywords: | Peach Ca2 signal Laser Scanning Confocal Microscope(LSCM) Fluo-3 AM-ester |
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