藤本月季组织培养初报

以MS为基本培养基,附加不同浓度的6-BA、NAA、KT等植物生长调节剂,对藤本月季"莫扎特"品种的组培快繁技术进行了研究,同时在4-10月进行了试管苗移栽基质筛选试验.结果表明:适宜的诱导培养基为MS+6-BA0.5 mg/L+NAA0.05 mg/L,腋芽萌发率达220%,平均芽高为2.6 cm;增殖培养基以MS+KT0.5 mg/L+NAA0.5 mg/L效果最好,增殖系数达4.2,且芽生长健壮;最适宜的生根培养基为1/2MS+IBA0.1 mg/L,生根率可达95%,且根粗壮发达.在移栽试验中,最佳的移栽基质为蛭石,且每年适宜在5、6月和9月进行试管苗移栽,其移栽成活率高达90%~95%.

Preliminary report on tissue culture and rapid propagation of climbing rose

The effects of different concentrations of 6-BA,IAA,NAA,and KT were studied on the establishment of subcultures and rooting cultures of climbing rose(Rosa chinensis cv.Mozart).The results showed that the suitable induction culture media was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L,for the sprouting rate of axillary bud up to 220 % and average growth height of bud was 2.6 cm.The optimum medium for multiplication was MS+KT 0.5 mg/L+NAA 0.1 mg/L,with the multiplication coefficient up to 4.2,and the buds grew strongly.1/2MS+IBA 0.1 mg/L was the optimum medium for rooting,with rooting percentage was 95 %,the roots developed well.Transplanting survival rate was obviously high with vermiculite as media.The transplanting survival rate was up to 90 %～95 % in May,June and September.