| 板栗品种线粒体SSR遗传多样性分析 |
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| 引用本文： | 张先启,郭献平,刘玉芬,张国庆,王珊珊,刘妍,秦岭,曹庆芹. 板栗品种线粒体SSR遗传多样性分析[J]. 北京农学院学报, 2012, 27(2): 17-20 |
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| 作者姓名： | 张先启 郭献平 刘玉芬 张国庆 王珊珊 刘妍 秦岭 曹庆芹 |
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| 作者单位： | 北京农学院植物科学技术学院,北京,中国102206;北京农学院生物技术学院,北京,中国102206;北京农学院生物技术学院,北京,中国102206;北京农学院植物科学技术学院,北京,中国102206 |
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| 摘 要： | 本试验通过对来自水稻和马铃薯的16对具有多态性的线粒体SSR引物进行筛选,得到2对适用于板栗的具有多态性条带的线粒体SSR引物。利用这2对多态性引物对76个板栗品种进行遗传多样性分析。结果表明2个多态性SSR位点检测到4个等位基因,平均每个位点产生2.0个等位基因。应用NTSYS2.10软件中的UPG-MA方法,对数据进行聚类分析,获得板栗资源线粒体SSR聚类图。结果表明:在相似系数0.15处可以将板栗种质资源按亲缘关系分成3组。
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| 关 键 词： | 线粒体SSR 板栗 遗传多样性 |
Genetic Diversity of Castanea mollissima Variety Based on Mitochondrial SSR Markers |
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| ZHANG Xian-qi , GUO Xian-ping , LIU Yu-feng , ZHANG Guo-qing , WANG Shan-shan , LIU Yan , QIN Ling , CAO Qing-qin. Genetic Diversity of Castanea mollissima Variety Based on Mitochondrial SSR Markers[J]. Journal of Beijing Agricultural College, 2012, 27(2): 17-20 |
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| Authors: | ZHANG Xian-qi GUO Xian-ping LIU Yu-feng ZHANG Guo-qing WANG Shan-shan LIU Yan QIN Ling CAO Qing-qin |
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| Affiliation: | b (a.Plant Science and Technology College,b.College of Biotechnology,Beijing University of Agriculture,Beijing,China,102206) |
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| Abstract: | The genetic diversity of 76 Castanea mollissima accesions was analyzed by mitoch ondrial SSR.Two polymorphic mtSSR primers were obtained by testing universal 16 primers from rice and potato.Two polymorphic mtSSR primers generated 4 alleles with an average of 2.0 alleles per primer among 76 C.mollissima accessions.Unweighted pair-group arithematic averages method(UPGMA) was applied to construct a cluster tree of C.mollissima resources based on mitochondrial SSR data by NTSYS2.10 software was.The results showed that 76 C.mollissima cultivars were classified into three groups at the level of similarity 0.15. |
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| Keywords: | mitochondrial SSR Castahea mollissima genetic diversity |
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