小麦条锈菌中国鉴别寄主洛夫林10和洛夫林13抗条锈性遗传研究

 洛夫林10和洛夫林13是中国小麦条锈菌重要鉴别寄主,为明确其抗条锈性遗传基础,本文采用常规杂交分析和等位性检测相结合的方法,将洛夫林10、洛夫林13分别与完全感病品种铭贤169杂交、自交和测交,获得各组合的正反交群体,在温室对其进行苗期抗性鉴定和统计分析,并分别与已知基因载体品系杂交和自交进行等位性检测。结果表明,洛夫林10对CYR17和CYR26的抗性分别由1对显性基因控制,属核遗传,洛夫林10抗CYR17和CYR26的基因与Moro、洛夫林13、K733所含的抗条锈病基因等位或紧密连锁;洛夫林13对CYR17和Su-1的抗性均由2对显性互补基因控制,对CYR26的抗性由1对显性和1对隐性重叠或独立基因控制,均属核遗传,洛夫林13抗CYR17、CYR26、Su-1的2对基因中有1对基因与Moro中的抗性基因等位或紧密连锁,抗CYR17的另1对基因与Hybrid46中的抗性基因等位或紧密连锁。表明洛夫林10和洛夫林13同含Yr9,洛夫林10的Yr9可能来源于无芒1号,洛夫林13的Yr9和另1对基因均来源于Skorospelka3B,未知基因可能位于6B或6A染色体上。

Genetic analysis of resistance to Puccinia striiformis f. sp. tritici of Chinese differential hosts Lovrin 10 and Lovrin 13

Lovrin 10 and Lovrin13 were used to differentiate races of Puccinia striiformis f. sp. tritici (Pst) in China. To unravel the genetic basis of resistance of Lovrin 10 and Lovrin 13 against Pst, we performed conventional hybridization analysis and allelism test. Lovrin 10 and Lovrin 13 were crossed with cultivar Mingxian 169 and cultivars with different resistance genes against Pst. Seedlings of the parents, BC₁, F₁ and F₂ progeny were evaluated for resistance to different Chinese Pst races. The results showed that the resistance of Lovrin 10 to CYR17 and CYR26 was controlled by one dominant gene which was nuclear inheritance, respectively. The resistance genes of Lovrin 10 against CYR17 and CYR26 maybe allelic or closely linked with the genes from Moro, Lovrin 13 and K733. The resistance of Lovrin 13 to CYR17 and Su-1 was controlled by two complementary dominant genes, whereas its resistance to CYR26 was controlled by one dominant gene and one overlapped or independently recessive gene. These genes all were inherited by nucleolus. One of the resistance genes of Lovrin13 against CYR17, CYR26 and Su-1 was allelic or closely linked with the resistance gene in Moro. The other resistance gene against CYR17 was allelic or closely linked with the resistance derived from Hybrid 46. Our results suggested that both Lovrin 10 and Lovrin 13 harbored the resistance gene Yr9. Yr9 gene in Lovrin 10 may be originated from Wumang No.1. However, Yr9 gene and the other unknown gene in Lovrin 13 may be originated from Skorospelka 3B and may be located on chromosome 6B or 6A.