普通小麦-华山新麦草易位系H9015-17抗条锈病基因的标记定位

采用我国当前流行的小麦条锈菌小种和重要致病类型,在常温条件下对普通小麦-华山新麦草易位系H9015-17进行苗期抗条锈性鉴定,并用当前主要流行小种CYR32对H9015-17与铭贤169的杂交后代及其双亲进行抗条锈性遗传分析,以揭示H9015-17抗条锈性遗传基础。结果显示,H9015-17对小麦条锈菌小种CYR31、CYR32、CYR33和致病类型Su11-4、Su11-7、V26、Su11-11均有良好的抗病性,对当前主要流行小种CYR32的抗病性由1对显性基因控制,暂命名为Yr Hua1。采用分子标记定位技术,筛选到5个与抗病基因Yr Hua1连锁的RGAP标记(M1、M2、M3、M4和M5)和1个SSR标记(Xgwm292),这些标记与抗病基因Yr Hua1的遗传距离分别为17.3、15.7、13.1、3.3、2.9和11.2,并将基因Yr Hua1定位在小麦染色体5DL上。研究结果将为分子标记辅助选择改良小麦抗条锈性提供宝贵的种质材料,建议在抗病育种加以利用。

Molecular mapping of a stripe rust resistance gene of wheat translocation line H9015-17 derived from Psathyrostachys huashanica Keng

To reveal the wheat stripe rust resistance mechanism of H9015-17, it was inoculated with seven prevalent races or strains of Puccinia striiforms f. sp. tritici (Pst) distributed widely in China, H9015-17, susceptible cultivar Mingxian169 and their progeny plants of cross combinations were tested with races CYR32. The methods of resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) markers were used to map the resistance gene(s) in its wheat genome. The results showed that H9015-17 expressed higher level disease resistance to seven races or strains of Pst. Genetic analysis showed that resistance gene of H9015-17 to CYR32 was controlled by one dominant gene, temporarily designated as YrHua1. The RGAP markers including M1, M2, M3, M4, M5 and SSR marker Xgwm292 were tightly linked to the gene YrHua1 and their genetic distance to YrHua1 were 17.3, 15.7, 13.1, 3.3, 2.9 and 11.2 cM, respectively. The gene was mapped to the long arm of chromosome 5D by SSR marker. Our research results provided a useful germplasm for marker-assisted selection used in wheat resistance breeding against stripe rust.