假禾谷镰刀菌LAMP快速检测方法的建立

<正>假禾谷镰刀菌(Fusarium pseudograminearum)1999年由Aoki等1]在澳大利亚首次发现,是引起冠腐病和赤霉病的主要病原,该病菌在世界小麦产区造成严重的经济损失,包括澳大利亚、北美、土耳其、和新西兰等地区,在澳大利亚冠腐病对小麦生产造成的损失超过了5 600万美元。特别在昆士兰、威尔士南部和澳大利亚南部的部分地区发生尤为严重。2011年Li等2]在中国河南省矮抗58小

Loop mediated isothermal amplification assay for sensitive and rapid detection of Fusarium pseudograminearum

Scab and crown rots caused by Fusarium pseudograminearum severely impact wheat production, and thereby establishing a quick and sensitive detection method is meaningful. In this study, a loop mediated isothermal amplification (LAMP) assay was developed for detection of F. pseudograminearum. In this procedure, a set of four primers matching a total of six sequences in the EF1-A gene region of F. pseudograminearum was synthesized for the LAMP assay by using genomic DNAs extracted from the mycelia. The optimum reaction temperature and assay time were determined, and the specificity of the LAMP assay was tested. This simple and sensitive method has a potential for detection of F. pseudograminearum in samples collected from the field.