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绿粘帚霉(Gliocladium virens)厚垣孢子的生物学特性
引用本文:谯天敏,朱天辉,李芳莲,刘富平,张毓红.绿粘帚霉(Gliocladium virens)厚垣孢子的生物学特性[J].四川农业大学学报,2006,24(2):166-170.
作者姓名:谯天敏  朱天辉  李芳莲  刘富平  张毓红
作者单位:1. 四川农业大学,林学园艺学院,四川,雅安,625014
2. 南川市林业局,重庆,南川,408400
摘    要:主要研究绿粘帚霉厚垣孢子的诱导因素及其生物学特性。营养条件和生态因子试验表明,PD为绿粘帚霉产厚垣孢子的最佳培养基,厚垣孢子产生的最适培养条件为:光照、振荡、pH 3、30℃,在此条件下可完全抑制其分生孢子的产生,并在1周内可产生厚垣孢子8.00×106个/mL;厚垣孢子萌发试验显示,25℃、pH为6~8为绿粘帚霉厚垣孢子的最佳萌发条件,48 h时可达90%以上的萌发率,在5℃下贮存比较适宜。

关 键 词:绿粘帚霉  厚垣孢子  生物防治  生物学特性
文章编号:1000-2650(2006)02-0166-05
收稿时间:2006-03-01
修稿时间:2006年3月1日

Biological Characteristics of Chlamydospore Production by Gliocladium virens
QIAO Tian-min,ZHU Tian-hui,LI Fang-lian,LIU Fu-ping,ZHANG Yu-hong.Biological Characteristics of Chlamydospore Production by Gliocladium virens[J].Journal of Sichuan Agricultural University,2006,24(2):166-170.
Authors:QIAO Tian-min  ZHU Tian-hui  LI Fang-lian  LIU Fu-ping  ZHANG Yu-hong
Abstract:Factors influencing chlamydospore production of Gliocladium virens and characteristic of the spore are studied.The optimal medium for chlamydospore production of G.virens is potato-dextrose liquid which is screened out from 8 kinds of mediums.In PD medium,the optimum culture conditions are pH 3,30?℃,rotating in the light.The acid environment greatly enhances chlamydospore formation and inhibited production of conidia entirely,and the numbers of chlamydospore production are 8.00×10~6?chlamydospores/mL in these conditions for a week;Studies of chlamydospore germinating show: The optimum germination pH value was pH 6~8 and the optimum temperature of germination is 25?℃;the germinating rate can reach above 90% after 48 hours;the optimal temperature for storing is under 5?℃.
Keywords:Gliocladium virens  chlamydospore  biological control  biological characteristics
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