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| 磺胺二甲嘧啶残留检测免疫传感器的感受器部件研制 | |
| 引用本文： | 高月林,李继昌,徐倩倩,霍贵成,刘丽波.磺胺二甲嘧啶残留检测免疫传感器的感受器部件研制[J].东北农业大学学报,2009,40(4). |
| 作者姓名： | 高月林 李继昌 徐倩倩 霍贵成 刘丽波 |
| 作者单位： | 1. 东北农业大学动物医学学院,哈尔滨,150030;黑龙江农业职业技术学院动物科学系,黑龙江,佳木斯,154007 2. 东北农业大学动物医学学院,哈尔滨,150030 3. 东北农业大学乳品科学教育部重点实验室,哈尔滨,150030 |
| 基金项目： | 乳品科学教育部重点实验室主任基金 |
| 摘 要： | 试验研究了用于检测磺胺二甲基嘧啶(SM2)残留的免疫生物传感器的最关键组成,即感受器部件的制备方法、最佳检测条件,并对所制备感受器部件的性能进行了测试。利用二醋酸纤维素作为载体支架结构,戊二醛为交联剂,己二胺为制孔剂,制备了最佳条件符合标准的载体膜。采用双盲评分法测定其性能,选取性能良好的载体膜包被SM2抗体制成抗体膜。将酶标抗原和SM2标准品联到抗体膜上,应用溶解氧分析仪测定体系中过氧化氢减少量。结果表明,质控膜和测定膜的电流变化值差异显著(P<0.05),感受器部件反应灵敏,最低检测限达5μg·L-1。 |
| 关 键 词： | 磺胺二甲嘧啶 残留检测 免疫传感器 感受器 |
Development of receptor of the immunosensor for detecting SM_2 residues |
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| GAO Yuelin,LI Jichang,XU Qianqian,HUO Guicheng,LIU Libo.Development of receptor of the immunosensor for detecting SM_2 residues[J].Journal of Northeast Agricultural University,2009,40(4). | |
| Authors: | GAO Yuelin LI Jichang XU Qianqian HUO Guicheng LIU Libo |
| Institution: | 1.College of Veterinary Medicine;Northeast Agricultural University;Harbin 150030;China;2.Animal Science Department;Heilongjiang Agricultural Professional Technical Institute;Jiamusi Heilongjiang 154007;3.Key Laboratory of Dairy Science;Ministry of Education;China |
| Abstract: | In this experiment,the method of preparation and the best detecting condition of the receptor,which is the key part of immunosensor for detecting the residue of Sulfamethazine(SM2) in agricultural products were studied,meanwhile the performance of the receptor was evaluated.Aceticacid cellulose was used as carrier structure,glutaraldehyde as cross-linking agent,hexamethylen diamine as cavity preparation reagent,the perfect and standard carrier membrane was prepared,and its performance was determined by doub... |
| Keywords: | sulfamethazine(SM2) residue detection immunosensor receptor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |