巨桉EXP基因家族的生物信息学分析

目的]本研究有助于了解EXP基因家族的基本特征,为深入研究其功能搭建平台。方法]本研究对从巨桉(Eucalyptus grandis Hill)中筛选出35个EXP基因家族成员(Egr EXP1 Egr EXP35),利用生物信息学方法对其基因特征与表达模式进行综合分析。结果]巨桉EXP基因分布在8条染色体之上,EXP蛋白均定位在细胞质膜上发挥作用,大多数的家族成员具有信号肽。巨桉EXP编码的蛋白质由α-螺旋、延伸链、无规卷曲、β-转角组成。进化分析结果表明,巨桉EXP蛋白与毛果杨(Populus trichocarpa) EXP蛋白的进化关系接近。35个巨桉EXP基因在巨桉未成熟木质部、成熟叶片、韧皮部、茎尖、木质部以及幼叶组织中表达模式存在显著差异。结论]EXP基因家族各成员的表达模式不同,Egr EXP17、Egr EXP18可能在巨桉木材形成过程中起重要作用。

Bioinformatics Analysis of EXP Gene Family in Eucalyptus grandis

Objective] To understand the basic features of EXP gene family and build a platform for further research on its function.Method] The Bioinformatics methods were used to analyze 35 EXP gene family members of the genome-wide sequence in Eucalyptus grandis (EgrEXP1~EgrEXP35).Result] The result indicated that E. grandis EXP genes located in 8 different chromosomes, and the subcellular localization were located on the cytoplasmic membrane. Most of the family members had a signal peptide. The proteins encoded by the E. grandis EXP genes were composed by alpha helix, extending chain, random coil and beta corner. Phylogenetic analysis revealed that E. grandis EXP protein shared close relationship with those in Populus trichocarpa. The 35 E. grandis EXP expression patterns were significantly different in the tissues including immature xylem, mature leaf, phloem, shoot tips, xylem and young leaf.Conclusion] The EXP expression pattern of E. grandis was different among family members. It could be inferred that EgrEXP17 and EgrEXP18 may play an important role in the process of wood formation in E. grandis.