容县乌榄叶挥发油化学成分及抗氧化活性分析

【目的】分析广西容县乌榄叶挥发油的化学成分及其抗氧化活性,为乌榄叶的药用开发提供参考。【方法】采用水蒸气蒸馏法提取广西容县乌榄叶中的挥发油,利用气相色谱—质谱联用法(GC-MS)分析其化学成分,通过峰面积归一法计算各成分的相对含量,并用DPPH·法对其抗氧化活性进行测定。【结果】容县乌榄叶挥发油共分离出55种成分,鉴定了53种,占挥发油总量的98.130%,其中单萜类化合物和倍半萜类化合物比例高,分别为20和18种;挥发油主要成分有雅槛兰-1(10),11-二烯(11.652%)、α-芹子烯(10.291%)、γ-依兰油烯(9.719%)、α-蒎烯(9.208%)和1,6-二甲基-4-异丙基-1,2,3,4,4a,7-六氢化萘(7.523%);乌榄叶挥发油质量浓度为1.2 g/L时,其对DPPH·的清除率为63%。【结论】容县乌榄叶挥发油化学成分种类较多,以单萜类化合物为主,且具有一定的抗氧化活性,可作为天然香料、抗氧化剂资源进一步研究。

GC-MS analysis and antioxidant activity of volatile oil from leaves of Canarium pimela Leenh. in Rongxian county

Objective]The chemical constituents and antioxidant activity of the volatile oil from leaves of Canarium pimela Leenh. in Rong County were studied to provide references for its medicinal development. Method]The volatile oil was extracted by steam distillation, and then the composition of oil was analyzed by GC-MS. Chromatographic peak area normalization method was used to calculate the relative mass fraction of each ingredient . The DPPH· method was used to study its antioxidant activity . Result]55 compounds were isolated and 53 compounds were identified in the leaves that composed about 98.130% of the total volatile oils, in which the ratio of monoterpenoids and sesquiterpenes was relatively higher, 20 and 18 types, respectively. The principal chemical constituents of the volatile oil were ere-mophila-1 (10), 11-diene (11.652%),α-selinene (10.291%), γ-muurolene (9.719%), α-pinene (9.208%) and 1,2,3,4,4a,7-hexahydro-1,6-dimethyl-4-(1-methylethyl)-Naphthalene (7.523%). The scavenging ratio for the DPPH· radicals reached 63% at the concentration of 1 . 2 g/L . Conclusion]There are many chemical constituents in the volatile oil and the main compounds are monoterpenoids. The volatile oil shows certain antioxidant activity and can be further studied as a natural spice and antioxidant.