Diversity and seasonal variations of mycorrhiza and rhizosphere bacteria in three common plant species at the Slovenian Ljubljana Marsh |
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Authors: | Matev? Likar Marjana Regvar Ines Mandic-Mulec Bla? Stres Hermann Bothe |
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Institution: | (1) Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, Slovenia;(2) Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, Slovenia;(3) Botanical Institute, University of Cologne, Gyrhofstr. 15, D-50923 Cologne, Germany;(4) Present address: Department of Animal Sciences, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia |
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Abstract: | Interactions between plants and microorganisms can significantly affect plant health and productivity as well as ecosystem
functioning. Detailed knowledge of the tripartite relationships between plants, fungi, and bacteria, and their environment
is still limited. In the present study, the soils adjacent to three plant species (Cruciata laevipes, Mentha piperita, Equisetum arvense) in the Ljubljana Marsh and the bulk, plant-free soil were analyzed for their bacterial community structure in June and October
2006. The terminal restriction fragment length polymorphism analysis indicated a different bacterial community structure in
the rhizosphere and in bulk soil, however, with almost no seasonal changes between late spring and autumn samples and no apparent
impact of the three plant species. In addition, root colonization of the three plant species by arbuscular mycorrhizal fungi
(AMF) and dark septate endophytes (DSE) was microscopically assessed monthly from May until October 2006. A presumably accidental
correlation between monthly precipitation and the degree of arbuscule formation, with the latter lagging 1 month, was noted
for M. piperita, the most heavily colonized of the three plant species. With all three plants, the phosphorus content in roots correlated
positively with most AMF structures. Microsclerotia of DSE were mainly abundant in autumn samples. Fungal diversity in roots
was estimated using temporal temperature gradient gel electrophoresis separation of the fungal polymerase chain reaction products
obtained for both 18S-rDNA and the 5.8S-ITS2-28S rDNA segments. No specific effects of either plant species or seasonal changes
on mycorrhizal community structure were discernible. |
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Keywords: | Arbuscular mycorrhizal fungi in wetlands Eubacterial communities in wetlands Microbial community analysis by molecular techniques Rhizosphere microorganisms Ljubljana Marsh |
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