|
|||||
|
|
| 不同发酵条件对苹果轮纹病拮抗细菌生长的影响 | |
| 引用本文： | 杨华,纪明山,李广旭,张广仁.不同发酵条件对苹果轮纹病拮抗细菌生长的影响[J].果树学报,2007,24(6):799-802. |
| 作者姓名： | 杨华 纪明山 李广旭 张广仁 |
| 作者单位： | 1. 沈阳农业大学植物保护学院,沈阳,110160;辽宁省果树科学研究所,辽宁营口,115009 2. 沈阳农业大学植物保护学院,沈阳,110160 3. 辽宁省果树科学研究所,辽宁营口,115009 |
| 摘 要： | 对1株苹果轮纹病细菌No.1菌株的发酵条件进行了探索,确定了其最佳培养基组成及培养条件。单因素发酵试验和正交试验结果表明,在含酵母浸膏5g、蔗糖20g、蛋白胨10g的1000mL液体培养基中,菌体生长量最大;细菌生长所需的最佳碳源为淀粉,最佳氮源为酵母浸膏和蛋白胨;当温度为28℃,pH7.0,在250mL三角瓶中装40mL液体培养基,接种量体积分数为5%,培养时间96h时,菌体生长量最大;在酸性条件下(pH4),细菌几乎不生长。 |
| 关 键 词： | 苹果轮纹病菌 拮抗细菌 发酵条件 |
| 文章编号： | 1009-9980（2007）06-799-04 |
| 修稿时间： | 2007-04-20 |
Influence of different fermentable conditions on growth of antagonistic Bacillus sp. for Botryosphaeria berengeriana f. sp. piricola |
|
| YANG Hua,JI Ming-shan,LI Guang-xu,ZHANG Guang-ren.Influence of different fermentable conditions on growth of antagonistic Bacillus sp. for Botryosphaeria berengeriana f. sp. piricola[J].Journal of Fruit Science,2007,24(6):799-802. | |
| Authors: | YANG Hua JI Ming-shan LI Guang-xu ZHANG Guang-ren |
| Institution: | 1 College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning 110161 China; 2 Liaoning Fruit Research Institute Yingkou, Liaoning 115009 China |
| Abstract: | The fermentable conditions of antagonistic bacteria No.1 strain were studied. The composition of fermentation medium and cultural conditions were confirmed. The single factor and orthogonal experiment indicated that the optimal cultural medium was a liquid medium (yeast extract 5 g,sucrose 20 g,protein peptone10 g). The most suitable carbon source was starch. The most suitable nitrogen sources were peptone and yeast extract; the optimal culture conditions were 28 ℃,pH 7.0,40 mL/250 mL flask of culture volume,96 h culture time and 5% inoculated volume. At pH 4 the antagonistic bacteria almost not grew. |
| Keywords: | Botryosphaeria berengeriana f sp piricola Antagonistic bacteria Fermentation condition |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |