柴油降解菌的筛选鉴定及降解特性研究

以柴油为唯一碳源,在富集、驯化培养基础上,从胜利油田石油污染土壤中筛选出一株柴油降解微生物WS14,通过外观形貌、Biolog鉴定等生理生化分析以及16S rRNA基因序列分析,确定该微生物为不动杆菌属（Acinetobacter sp.）。通过对筛选到柴油降解菌的生长因子研究发现：适合WS14菌株生长的最佳pH值为6.0～8.5,适宜温度为35℃,最佳培养时间为72h,并且柴油含量为11.5%时菌株的生长量最大;实验结果显示,WS14菌株对高盐和高含量柴油具有一定的耐受性,能够在5%含盐量和30%柴油含量的环境生长。

Screening and Identification of Diesel Fuel Degrading Microorganism

The diesel fuel degrading bacteria used in this study was isolated from the aged oily sludge of Shengli Oil Field,and tentatively identified as Acinetobacter sp.through morphological and Biolog microplate analysis,as well as 16S rRNA sequencing.The strain,designated as WS14,was further characterized for its ability to degrade diesel fuel through determining the optical density（OD600）,and the results of growth curves showed that the optimum condition was at pH 6.0～8.5,diesel concentration of 11.5%,and the inoculated temperature and time of 35 ℃ and 72 h,respectively.In addition,the WS14 strain had capacity to grow in 5% salty and 30% diesel containing medium.The WS14 strain was proposed a potential biodegradation for diesel oil and the biodegradation efficiencies varied as following 58.6%,35.4%,18.2% for 1%,5% and 10% diesel concentration,respectively.In addition,the chromatographic fingerprints indicated that the alkenes with less carbon numbers were preferentially degraded.