| 犬细小病毒VP2主要抗原表位区的原核表达载体构建及诱导表达 |
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| 引用本文： | 马辉,赵绪永. 犬细小病毒VP2主要抗原表位区的原核表达载体构建及诱导表达[J]. 郑州牧业工程高等专科学校学报, 2012, 32(2): 9-11 |
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| 作者姓名： | 马辉 赵绪永 |
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| 作者单位： | 郑州牧业工程高等专科学校生物工程系,河南郑州,450011 |
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| 摘 要： | 利用PCR扩增CPV-2bVP2蛋白的主要抗原表位区基因，并将该基因片段克隆到原核表达载体pGEX-6P-1上，通过诱导表达条件的摸索，实现了VP2蛋白主要抗原表位区在大肠杆菌中的高效表达，sDs—PAGE检测表达的融合蛋白主要以可溶形式存在，并利用亲和层析得到融合蛋白。
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| 关 键 词： | 犬细小病毒 VP2 原核表达 亲和纯化 |
Construction of pGEX-VP2 prokaryotic expression vector and expression of the major epitope domain of canine parvovirus VP2 |
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| MA Hui,ZHAO Xu-yong. Construction of pGEX-VP2 prokaryotic expression vector and expression of the major epitope domain of canine parvovirus VP2[J]. Journal of Zhengzhou College of Animal Husbandry Engineering, 2012, 32(2): 9-11 |
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| Authors: | MA Hui ZHAO Xu-yong |
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| Affiliation: | (Department of Bioengineering of Zhengzhou College of Animal Husbandry Engineering,Zhengzhou Henan 450011,China) |
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| Abstract: | To establish a prokaryotic expression system expressing major epitope domain of canine parvovirus VP2 protein to serve the diagnosis of CPV,the fragment of CPV-2b VP2 gene was cloned from cloned vector by PCR.Then,recombinant E.coli strain BL-21 was induced to express the fusion protein by IPTG.The expressed productions were detected by SDS-PAGE and western blot,and the results showed that the VP2 protein was efficiently expressed and the size of recombinant protein is 34KD according to being expected. |
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| Keywords: | CPV VP2 gene prokaryotic expression affinity purification |
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