A proposed method of polyacrylamide gel electrophoresis in acid environments applied to gliadins of wheat grains |
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Authors: | A Houwing Frieda van Dreven |
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Institution: | (1) RIVRO, P.O. Box 32, NL 6700 AA Wageningen, the Netherlands |
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Abstract: | Summary In this paper we describe the method of wheat gliadin electrophoresis in use at RIVRO, Wageningen. It differs from other techniques mainly by the application of an alternative buffer system, making it possible to polymerise the gels in a buffered alkaline environment and to perform the run at pH 3.1 without extensive buffer changing steps. Advantages are a greater gel reproducibility and the ease of gel handling. Furthermore, a rationalised protein extraction procedure, a cheap shaking system for staining baths and a better (slower moving) tracking dye are described. |
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Keywords: | Triticum aestivum wheat electrophoresis system acid polyacrylamide gel gliadins variety identification |
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