| 茶叶中硫丹及硫丹硫酸酯的ELISA快速测定 |
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| 引用本文： | 吕潇,任凤山,王文博,杜红霞,李慧冬,丁蕊艳,朱端卫,LEE Nanju A,SHEILD Ross,KENNEDY Ivan R.茶叶中硫丹及硫丹硫酸酯的ELISA快速测定[J].茶叶科学,2006,26(2):141-146. |
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| 作者姓名： | 吕潇 任凤山 王文博 杜红霞 李慧冬 丁蕊艳 朱端卫 LEE Nanju A SHEILD Ross KENNEDY Ivan R |
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| 作者单位： | 1. 华中农业大学资源与环境学院,湖北 武汉 430070;2. 山东省农业科学院中心实验室,山东 济南 250100;3. Department of Food Science & Technology, University of NSW, Sydney, NSW, 2050, Australia;4. Agrisearch Analytical, Sydney NSW 2039, Australia;5. Faculty of Agriculture, Food and Natural Resources, University of Sydney, Sydney, NSW, 2006, Australia |
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| 摘 要： | 研究应用酶联免疫方法,快速检测茶叶中硫丹及硫丹硫酸酯。茶叶提取后,不净化,用PBS缓冲液稀释500倍后,进行酶联免疫方法测定,方法平均回收率为86.6%~91.1%;经SPE净化后,回收率68.7%~123%。标准曲线IC50为2βµg/L,线性范围1~10βµg/L。直接提取法最低检测限2βmg/kg,净化法为0.06βmg/kg。经气相色谱/质谱联用方法验证,灵敏度、精密度和准确度均符合快速检测要求。该法具有快速、简便、低耗、易操作等特点。
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| 关 键 词： | 硫丹 酶联免疫 茶叶 气相色谱/质谱联用仪 |
| 文章编号： | 1000-369X（2006）02-141-06 |
| 收稿时间： | 2005-12-12 |
| 修稿时间： | 2006-01-12 |
Determination of Endosulfan and Endosulfan Sulfate in Tea by ELISA |
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| LU Xiao,REN Feng-shan,WANG Wen-bo,DU Hong-xia,LI Hui-dong,DING Rui-yan,ZHU Duan-wei,LEE Nanju A,SHEILD Ross,KENNEDY Ivan R.Determination of Endosulfan and Endosulfan Sulfate in Tea by ELISA[J].Journal of Tea Science,2006,26(2):141-146. |
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| Authors: | LU Xiao REN Feng-shan WANG Wen-bo DU Hong-xia LI Hui-dong DING Rui-yan ZHU Duan-wei LEE Nanju A SHEILD Ross KENNEDY Ivan R |
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| Institution: | 1. Faculty of Natural Resources & Environment, Huazhong Agricultural University, Wuhan 430070, China;2. Central Laboratory, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China;3. Department of Food Science & Technology, University of NSW, Sydney, NSW, 2050, Australia;4. Agrisearch Analytical, Sydney, NSW, 2039, Australia;5. Faculty of Agriculture, Food and Natural Resources, University of Sydney, Sydney, NSW, 2006, Australia |
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| Abstract: | A rapid method based on Enzyme-linked immunosorbent assay (ELISA) was developed for determination of endosulfan isomers and endosulfan sulfate in commercial black and green tea. The average recoveries obtained from crude extracts ranged from 86.6% to 91.1%, which from purified extracts ranged from 68.7% to 123%. The liner range of the standard curve was from 1 μg/L to 10 μg/L. The detection limit of crude extract was 2 mg/kg and the purified was 0.06 mg/kg. This study showed a validation of endosulfan analyzed in tea by ELISA and GC-MS at different levels and proved that ELISA is a quick, low cost and practical technique for monitoring pesticides in tea. |
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| Keywords: | endosulfan ELISA tea GC/MS |
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